摘要
环状芽孢杆菌A 1 3 83产细胞壁溶解酶的适宜的碳源为酵母葡聚糖 ,氮源采用蛋白胨与(NH4 ) 2 SO4 混合氮源 ,接种量 6%～ 1 0 % ,2 5 0mL三角瓶装液量为 40mL ,发酵温度为 3 0～ 3 5℃ ,培养基初始pH 6 5～ 7 0。在此条件下发酵 5 4h后可达到最高酶活 74 6u/mL ,比条件优化前的酶活提高了 3 8 7%。将所得的发酵液用于红法夫酵母虾青素的酶法提取 ,总类胡萝卜素的提取率达到 96%以上。
The effects of different factors on lytic enzyme production by Bacillus circulans A 1.383 were studied in flask. It is found that water insoluble yeast glucan was the best C sources and the mixture of peptone and (NH_4)_2SO_4 was the best N sources. It is also seen that the best inoculum concentration was 6%～10% with 40?mL medium volume in 250?mL flask for B. circulans A 1.383 to produce lytic enzyme with high activity. The fermentation temperature was 30～35℃ and the initial pH value of growth medium was 6.5～7.0 in this paper. The lytic enzyme activity of fermentation fluid reached 74.6?u/mL and it is increased by 38.7% compare with the initial. The fermentation fluid was used for extracting astaxanthin of Phaffia rhodozyma and over 96% of the total carotenoid could be extracted within 36 h when reaction temperature was 30℃ and pH was 6.5.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2004年第10期21-25,31,共6页
Food and Fermentation Industries
基金
广东省科技计划项目 (2 0 0 2C10 4 0 10 1)
华南理工大学自然科学基金 (E5 32 12 77)
广州市科技计划项目(2 0 0 3Z2 -E0 131)资助
