摘要
目的 研究鱼藤酮慢性中毒大鼠尾核脑区及血浆的一氧化氮 (NO)含量和一氧化氮合成酶 (NOS)活性的变化规律。方法 Wistar大鼠每日皮下注射 0 3 3 5、0 670、1 0 0 5mg kg鱼藤酮共 90d。于 3 0、60、90d处死动物 ,分离血浆和尾核脑组织 ,以生化方法测定NOS活性和NO含量。结果 在鱼藤酮中毒 3 0、60、90d 3个时相点 ,大鼠血浆和尾核脑组织中NOS活性和NO含量增加 ,增加程度因中毒剂量的提高而升高、随中毒时间的延长而升高。
Objective To observe the rotenone induced changes of nitric oxide (NO) concentration and nitric oxide synthase (NOS) activity in rat caudate nuclei and blood plasma. Methods Wistar rats were daily administrated with rotenone at the doses of 0.335, 0.670 and 1.005 mg/kg(sc). Animals were sacrificed on days 30, 60 and 90. The NO concentration and the NOS activity in the caudate nuclei and blood plasma were determined by biochemical methods. Results At each time point of days 30, 60 and 90, rotenone induced significant increases in NO concentration and NOS activity in both the caudate nuclei and blood plasma in dose and time dependent manners. Conclusion Long term exposure to rotenone leads to the increase of nitric oxide and nitric oxide synthase in brain tissues and blood plasma in rat.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第13期1131-1133,共3页
Journal of Third Military Medical University
基金
重庆市科学研究基金课题 ( 2 0 0 0 )
