摘要
为进一步研究基因疫苗的免疫机制与免疫效果,探讨外源基因在乳腺中的定位表达,应用重组PCR方法,将猪瘟病毒(CSFV)E2基因与牛β-乳球蛋白(BLG)5′部分调控序列连接起来,并插入到真核表达载体pEGFP-C1上,构建了含有CSFVE2基因的乳腺特异表达载体。
The mammary-specific expression vector of pEBE was constructed with pEGFP-C1 and BE fragment.The BE fragment was gained with bovine beta-lactoglobulin (BLG) 5′ regulation region and the E_2 gene of classical swine fever virus (CSFV) by the method of recombinent PCR.The results shows that we could successfully get a DNA vaccine of CSFV E_2 gene that can be expressed in mammary gland cell in order to study the effect of DNA vaccine and mammary bioreactor.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2004年第11期11-14,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
科技部863高技术研究发展计划项目(2001AA213081)
关键词
猪瘟病毒
E2基因
表达载体
基因疫苗
牛β-乳球蛋白
classical swine fever virus
E_2 gene
bovine β-lactoglobulin (BLG)
expression vector
