摘要
目的观察大鼠脑冷冻伤后脑组织中一氧化氮合酶(NOS)和血浆一氧化氮(NO)的变化,探讨氯胺酮对NO的影响.方法49只SD大鼠随机分为假手术组、未治疗组和氯胺酮治疗组.分别于2、6、24 h处死动物,0℃～4℃取脑,匀浆离心制成10%组织匀浆,用分光光度法测脑组织NOS活性和血浆NO量.结果脑组织中NOS水平在冷冻伤后2 h明显升高,并随时间延长呈上升趋势.血浆NO量也有相应改变.氯胺酮治疗后脑组织NOS水平和血浆NO量在2、6 h较未治疗组显著下降,24 h无差异.结论氯胺酮能降低冷冻伤周围脑组织中NOS水平和血中NO量.
Objective To observe the change of nitric oxide synthase(NOS) in the rat brain and nitric oxide(NO) in plasma and to study the effects of treatment with ketamine on nitric oxide after cold-induced injury. Methods Forty-nine Sprague-Dawley rats were divided into three groups:sham-operated group, no treatment group and ketamine treatment group, respectively. After death at 2, 6 and 24 hours, cortical slices were taken adjacent to the lesion on the traumatized hemisphere and from comparable sites in the contralateral hemisphere, tissues were homogenized and centrifuged. NOS activity and NO output were assayed with spectrophotometric analysis. Results NOS and NO were increased after head trauma. In groups with ketamine treatment 1 hour after trauma, NOS levels and plasma NO reduced at the 2nd and the 6th hour respectively. Conclusion Our conclusion is that 180 mg/kg IP ketamine is effective in reducing NOS level and plasma NO after head cold injury.
出处
《苏州大学学报（医学版）》
CAS
2004年第5期640-642,共3页
Suzhou University Journal of Medical Science
关键词
冷冻伤
一氧化氮
氯胺酮
NMDA受体
大鼠
cold injury
nitric oxide
ketamine
N-methyl-D-aspartate receptor
rat
