摘要
目的 :了解 TORCH中巨细胞病毒 (CMV)、风疹病毒 (RV)、弓形体 (TOX)及单纯疱疹病毒(HSV)在住院新生儿中的感染情况及其血清细胞因子 IL- 4、IFN- γ和 TNF- α的变化。方法 :应用 EL ISA法检测6 5例住院新生儿血清 CMV- Ig M、TOX- Ig M、RV- Ig M和 HSV2 - Ig M,对检出的 18例 TORCH- Ig M阳性患儿及正常对照组 15例出生正常的新生儿脐血 (已检测 TORCH- Ig M为阴性 ) ,同样用 EL ISA法检测其血清中细胞因子IL- 4、IFN- γ和 TNF- α的含量。结果 :6 5例住院新生儿中 CMV- Ig M阳性 14例 ,TOX- Ig M阳性 8例 ,HSV2 - Ig M阳性 5例 ,RV- Ig M阳性 3例。感染率分别占同期住院新生儿的 2 1.5 4 %、 12 .31%、 7.6 9%及 4 .6 2 %。TORCH-Ig M阳性组 (18例 ) IL- 4、 IFN- γ和 TNF- α的含量分别为 (2 0 .4 7± 2 .5 5 )、 (2 92 .6± 73.9)和 (2 19.11±2 2 .12 7) ng· L- 1 ,正常对照组分别为 (2 7.11± 8.97)、 (2 0 4 .5± 2 5 .7)和 (14 7.74± 2 0 .19) ng· L- 1。 TORCH阳性组与对照组相比血清中 IL- 4水平低下 (P<0 .0 5 ) ,而 TNF- α和 IFN- γ含量均明显升高 (P<0 .0 5 )。结论 :新生儿中 TORCH感染较普遍 ,其中以 CMV为主 ,TOX次之。TORCH感染后 ,免疫平衡遭到破坏 ,细胞因子分泌失衡 。
Objective To observe the TOROH(including CMV,RV,TOX,and HSV) infections of newborns in hospital and study the changes of the levels of IL-4, IFN-γ, and TNF-α, respectively,in serum. Methods The neonates were given serum TORCH-IgM tests by ELISA.The levels of IL-4, IFN-γ, and TNF-α were all detected by double antibody with filling ELISA. Results There were altogether 18 patients with positive TORCH-IgM of 65 neonates in hospital. Among them there were 14 patients with positive CMV-IgM, 8 with TOX-IgM, 5 with positive HSV 2-IgM, and 3 with positive RV-IgM. The infect rates were 21.54%,12.31%,7.69%, and 4.62%, respectively,among neonates in hospital at the same time .The levels of IL-4, IFN-γ, and TNF-α in patients with positive TORCH-IgM were 20.47±2.55,292.6±73.9,and 219.11±22.27 ng·L -1 ,respectively, while those in normal control group were 27.11±8.97,204.5±25.7, and 147.74±20.19 ng·L -1 ,respectively. The level of IL-4 of patients with positive TORCH-IgM decreased (P<0.05),the levels of IFN-γ and TNF-α increased (P<0.05), compared with control group. Conclusion TORCH infection is very common in neonates in hospital. The detection rate of positive CMV-IgM is the highest, TOX-IgM comes next. After TORCH infection, the balance of immune is destroyed, and cytokines are secreted imbalanced. In the TORCH infection treatment, the immunity adjusting treatment should be emphasized besides anti-pathogen therapy.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2004年第6期925-927,共3页
Journal of Jilin University：Medicine Edition
基金
吉林省计划经济委员会资助课题 (1999)
