摘要
目的 探讨氟吗啉的致突变性。方法 首先测定氟吗啉对V79和CHL的细胞毒性 ,然后在非代谢活化和代谢活化条件下 ,进行氟吗啉诱发V79细胞HGPRT基因突变试验和CHL细胞染色体畸变试验。结果 以氟吗啉 5 0 0、10 0、2 0和 4μg mL浓度处理V79细胞 ,处理组诱变率与阴性对照组突变率比较 ,差异无显著性 (P≥ 0 0 5 ) ;以 5 0 0、2 5 0、12 5和 62 5 μg/mL浓度处理CHL细胞 2 4、48h后 ,在非代谢活化条件下 ,处理组染色体畸变均小于 5 %,但在代谢活化条件下 ,处理组染色体畸变率均大于 5 %,而且呈剂量 反应关系 ,通过G 显带发现断裂集中发生于 4q上 ,是断裂热点。结论 可以认为应用氟吗啉 10 0～ 2 0 0mg/L防治植物病害不会对人类健康造成危害 ,但是职业人群应注意防护。
Objective To evaluate the mutagenesis of the flumorph.Methods In this study,the toxicity of the flumorph to the Chinese hamster lung cells (V79 and CHL) was tested,then in the presence and absence of the metabolic system,the V79 HGPRT gene mutation and the CHL chromosome aberration induced by flumorph were detected.Results The rates of the mutant clones from V79 cells treated by the dose level of 500,100, 20 and 4 μg/mL were similar to those of the negative control ( P >0.05). In the dose level of 500,250,125 and 62.5 μg/mL,the ratios of the chromosome aberration of CHL cells were less than 5% in the absence of the metabolic activation system,but the ratios of the chromosome aberration were more than 5% in the presence of the metabolic activation system in every dose level,and the ratios of the chromosome aberration went up with the increasing of the dose,which showed clear dose- response relations. We found that the most of breaks were located on the chromosome 4 q,which speculated that 4 q was the heat spot of the breaks by the G-band dye method. Conclusion We can think that flumorph could not damage the human health at the concentration of 100～200 mg/L to protest and treat the plant disease,but the professional crowd should be paid more attention.
出处
《卫生毒理学杂志》
CSCD
北大核心
2004年第3期151-153,共3页
Journal of Health Toxicology
