摘要
目的 研究骨架蛋白 β actin在氯丙烯引起的周围神经病中的作用。 方法 采用原代培养的大鼠大脑神经元 ,用相差显微镜观察细胞形态 ,同时用免疫组化方法分析测定细胞内 β actin含量的变化。 结果 氯丙烯使大脑神经元中 β actin含量显著下降 ,低剂量组降低 5 2 %,高剂量组降低 85 %(P <0 0 1)。 结论 提示氯丙烯急性中毒可导致大鼠大脑神经元中 β actin含量降低。
Objective To investigate the role of β-actin cytoskeleton in toxic peripheral neuropathy in human induced by allyl chloride(AC). Methods Used the methods of primary cell culture,the growth condition of cerebrum neurons was observed with phase contrast microscope.Immunohistochemistry technology was used to analyze β-actin in the primary cultured cerebrum neurons treated with AC for 24 hours. Results Immunohistochemistry analysis showed that β-actin was decreased significantiy in cerebram neurons of 0.6mmol/L and 1.2 mmol/L groups by 52% and 85% respectively( P <0.01). Conclusion The results suggest that AC can decrease the contents of β-actin of primary cultured cerebrum neurons.
出处
《卫生毒理学杂志》
CSCD
北大核心
2004年第3期131-133,共3页
Journal of Health Toxicology
基金
973(2 0 0 2CB51 2 90 7)
国家自然科学基金 (30 2 71 1 38)
山东省自然科学基金 (Y97C1 30 4 9)
