摘要
本文研究了Bowman-Cole土壤有机磷分组法应用于石灰性土壤存在的若干问题,并提出了改进法。改进法的四组土壤有机磷(P0)的分组为:在氯仿预处理土样后用0.5molL-1NaHCO3浸提活性P0;然后,先用0.05molL-1NaOH、后用1molL-1H2SO4处理残余土样浸提中度活性P0,0.05molL-1NaOH提取的无机磷和1molL-1H2SO4提取的有机磷属于中度活性P0;在氯仿和0.5molL-1NaHCO3处理后,用0.05molL-1NaOH浸提稳定性P0,把浸提液的酸度调至pH3.00时,上清液中的有机磷为中度稳定性P0,而沉淀中的有机磷为高稳性P0。结果表明,改进法能更好地区分具有不同矿化率的有机磷化合物,例如,核酸、甘油磷酸、植酸钙、肌醇三磷酸铁、植酸铁等。改进法增加了超声波处理,使震荡时间也明显缩短。因此,石灰性土壤有机磷分组的改进法比Bowman-Cole法更理想。
Some problems on Bowmen-Cole' fractionation method of organic phosphorus (P_0) in calcareous soil were studied. A modified fractionation scheme was proposed: The labile P_0 in which contained the microbial P_0 was extracted by fumigation of soil with chloroform pretreatment.The extracting agent concentration of NaOH for extracting stable P_0 was reduced to 0.05 mol L^(-1) NaON as suitable; when the extract by 0.05 mol L^(-1) NaON in which contained the stable P_0 was adjusted to pH=3.00, the supernatant solution contained moderately stable P_0, while the precipitate contained highly stable P_0.Moreover, the moderately labile P_0 was changed to be extracted by 1mol L^(-1) H_2SO_4 from the residual after pretreatment with 0.05mol L^(-1) NaOH to remove the stable P_0, and the inorganic P contained in the 0.05 mol L^(-1) NaOH belongs in moderately labile P_0 . The result of this study shows, the organic phosphorus compounds with different mineralization rate, such as nucleic acid, phospho-glycerol, calcium phytate, iron of inositol triphosphate and iron phytate, were more reasonably separated by the modified method.The shaking time for extracting soil organic phosphorus was shortened by ultrasonic treatment.
出处
《土壤通报》
CAS
CSCD
北大核心
2004年第6期743-749,共7页
Chinese Journal of Soil Science
基金
国家自然科学基金(No.48571041)
湖北省自然科学基金(No.95J39)资助
