摘要
丙型肝炎病毒(HCV)基因组是线性、正链RNA分子,其全长核苷酸(nt)序列约为10kb,由5′端(332nt)非编码区、3′端(54nt)非编码区和一长的开读框架(ORF:1～9033nt或9075nt、9097nt)3部分构成;根据编码区基因序列可推定出HCV多聚蛋白前体(3011aa)的组成。用计算机对基因组的核苷酸序列进行分析,结果表明HCV基因组具有较高的突变频率,因而基因序列呈现出较大的变异,其中以E_1区和E_2/NS_1区的变异性最高;相对而言,C区、NS_3～NS_5区和5′-NCR的核苷酸序列则有明显的保守性;同时将HCV和已知的其它病毒的基因组及多聚蛋白进行比较,结果显示在分类上HCV与黄病毒和瘟病毒有关。
Approximately 10 000 nucleotides (nt) are found in the positive-stranded RNA
genome of hepatitis C virus (HCV) which contains 5'untranslated region(UTR, 332nt),
3'-UTR(54nt) and a single large open reading frame (ORF, 9033nt) encoding a viral polypeptide
precursor of 3 011 amino acids. The computerized comparison of all the complete and partial
HCV sequences published to date indicates that HCV genome has a higher mutation frequency.
The E_1 and E_2/NS_1 genes show substantial nucleotide sequence variation, while the C
(nucleo-capsid protein), NS_3 and NS_4 genes all show greater sequence conservation among the
different HCV isolates. Furthermore, some partial homology exists between the sequence of both
the HCV genome and its encoded polypeptide and the sequences of human flaviviruses and animal
pestiviruses, suggesting that HCV may be a distant relative of flaviviruses and pestiviruses.
出处
《军事医学科学院院刊》
CSCD
北大核心
1993年第3期233-237,共5页
Bulletin of the Academy of Military Medical Sciences
关键词
丙型肝炎病毒
CDNA克隆
DNA序列
hepatitis C virus (HCV)
cDNA clone
DNA sequencing and its analysis
