摘要
目的 :获得大量的重组红豆杉苯丙基转移酶 (BAPT) ,为紫杉醇半合成代谢提供廉价的催化剂。方法 :根据DNA重组技术 ,构建原核表达载体pET -BAPT ,使目的基因位于原核T7启动子下游 ,IPTG诱导基因表达。结果 :BAPT高效表达 ,重组蛋白主要以包涵体的形式存在。结论 :为获得可溶性重组蛋白BAPT奠定了理论基础。
Objective:To obtain quantitive recombinant Phenylpropanoid CoA Acyltransferase of Taxus and provide cheap catalyst for the taxol semisynthesis.Methods:Based on DNA recombinant techniques,expression vector pET-BAPT was constructed.The target gene was downstream the T7 promotor and induced with IPTG.Results:BAPT was expressed effectively and existed in inclusion body of E.coli.Conclusions:This study is the basis for the supply of the soluble fusion protein BAPT.
出处
《生物技术》
CAS
CSCD
2004年第5期13-15,共3页
Biotechnology
基金
河北省自然科学基金资助项目 (30 0 1 62 )
