摘要
利用肝癌单克隆抗体(H_(10))制备CD_3×H_(10)双特异性抗体及其肝癌特异性靶向LAK 细胞(HT-LAK),结果表明:CD_3M_cA_b 封闭LAK 细胞后对其生化自发光有明显抑制作用,但细胞对rIL-2仍保持较高的反应性。为此我们选用CD_3M_cA_b 为杂交抗体组份。经制备的HT-LAK 细胞在低效靶比(0.1:1;1:1)时对H7402肝癌细胞有较高的杀伤活性,明显高于单纯LAK 细胞(P<0.01),但在高效靶比(5:1:10:1)时二者无明显差异。
In order to more effectively target the lymphokine-activated killer(LAK)
cells to the tumor cells,the bispecific antibody-armed LAK cell method was used.At
first,we prepared two specific monoclonal antibodies,one recogize the human hepatoma antigen H10 and the other for the CD_3 molecules on human T lymphocytes.Then the
monoclonal antibodies were respectively digested with pepsin and F(ab')2 were purified by
Chromotography.Furthermore,by using chemical reduction and cross-linking method,the
two different specific Fab' fragments were prepared and linked with each other to form
the bispecific hybrid antibody.The LAK cells were incubated with the bispecific hybrid
antibody in room temperature for 30 minutes.The chemical Iumination result showed that
bispecific antibody specifically bind with the LAK cells.In proliferation assay,the
bispecific antibody armed LAK cells were still highly responsive to recombinant human
interleukin-2(IL-2).Finally,the cytotoxic effect of the armed-LAK cells on human hep-
atoma cells was assayed,t was shown that the armed-LAK cells exhibited significantly
higher cytotoxicity against human hepatoma cell line H7402 than that of the non-armed
LAK cells.Another human leukemic cell line K562 showed no competition the hepatoma
cells in binding with the armed-LAK cells.The results suggested that the bispecific
hybrid antibody-armed LAK cells were specifically human hepatoma targetting and showing
high antitumor effects.
出处
《江苏医药》
CAS
CSCD
1993年第8期408-411,共4页
Jiangsu Medical Journal
