摘要
目的 观察胆管内放射对平滑肌肌动蛋白 (SMA)在犬胆管损伤愈合过程中的表达影响 ,探讨10 3 钯 (10 3 Pd)放射性支架抑制胆管损伤后狭窄的作用及意义。方法 通过犬肝外胆管损伤模型将10 3 Pd放射性支架和普通支架分别植入犬肝外胆管内 ,分别于手术后 30d取材行SMA免疫组织化学SP染色观察。结果 10 3 Pd放射性支架组犬胆管SMA表达较弱 ,而普通支架组却较强 ,但是10 3Pd放射性支架组犬胆管腔狭窄程度比普通支架组轻。结论 10 3 Pd放射性支架犬胆管内放射可以减弱SMA在胆管愈合过程中的表达 ,从而可以抑制胆管损伤后因胆管瘢痕性挛缩导致的胆管狭窄。
Objective To observe the effect of radiation on the expression of smooth muscle actin (SMA) in the bile duct during the healing process and the inhibitory function of 103palladium (Pd) radioactive stent on the stricture of bile duct after injury. Methods Twelve mongrel dogs were made models of bile duct injury: duodenotomy was performed, a balloon catheter was inserted into the general bile duct and saline with high pressure was perfused thereinto to cause laceration of the mucosa, and then the balloon catheter was withdrawn and ordinary alloy stent or 103Pd radioactive stent was inserted into the general bile duct. Thirty days after the dogs were killed. Their bile ducts were taken out to undergo HE staining to observe the area of general bile duct, thickness of the tunica intima, area of residual bile duct cavity, stricture degree, and circumference of bile duct. The expression of SMA in the bile duct tissue was detected by immunoistochemistry. Results SMA was expressed in 5 of the 6 specimens of bile duct in the 103Pd radioactive stent group and 2 of the 6 specimens of the ordinary stent group (P<0.01). The maximum thickness of tunica intima of general bile duct was 0.78 mm±0.12 mm in the 103Pd radioactive stent group, significantly less than that of the ordinary stent group (1.86 mm±0.14 mm, P<0.01). The percentage of maximum stricture area of the 103Pd radioactive stent group was 23% ±16%, significantly lower that that of the ordinary stent group (56%±22%, P<0.01). The circumference of bile duct cavity of the 103Pd radioactive stent group was 9.7 mm±1.6 mm, significantly longer that of the ordinary stent group ( 7.0 mm±1.4 mm, P<0.01). Conclusion 103Pd radioactive stent reduces the expression of SMA in the bile duct during the healing process, thus inhibiting the stricture of bile duct caused by scar contracture at the anastomotic stoma.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2004年第17期1475-1477,共3页
National Medical Journal of China
