摘要
目的 证实骨髓干细胞心肌内移植治疗对心脏功能的改善和促血管新生作用。方法 小型猪冠状动脉结扎制备心肌梗死模型 ,然后经冠状动脉内注射骨髓单个核细胞 ,术后 3周用超声心动图以及左心室造影检测心功能的变化 ,核素心肌显像观察心肌灌注的情况 ,冠状动脉造影观察侧支循环的形成 ,免疫组化计数血管密度。结果 心肌梗死小型猪冠状动脉注射骨髓单个核细胞后 ,左心室造影显示左室 +dP dtmax与对照组比较增高。核素心肌显像显示冠状动脉注射骨髓单个核细胞后心肌灌注显著改善。移植治疗后冠状动脉造影显示有侧支循环形成 ,血管密度计数比对照组增加了5 2 2 % (5 6 6± 11 7 mm2 vs 37 2± 8 4 mm2 ,P <0 0 5 )。结论 骨髓单个核细胞心肌内移植可能通过促进血管新生改善心脏收缩功能。
Objective To manifest the angiogenic effect and heart function improvement of transplantation therapy through the experiments of porcines. Methods The coronary arteries of porcines were ligated to create myocardial infarction and then the bone marrow mononuclear cells were injected into the coronary artery. Three weeks after transplantation the heart function was measured by echocardiography and left ventricular angiography. The myocardial blood perfusion was measured by single photon emission computerized tomography. The number of microvessels was also counted. Results After the injection of bone marrow mononuclear cells, left ventricular angiography showed the increasing of dP/dt. The myocardial blood perfusion measured by single photon emission computerized tomography increased. Collateral vessels occurred, and the number of vessels was higher than that of the control (56.6±11.7/mm2 vs. 37.2±8.4/mm2,P<0.05). Conclusion Bone marrow mononuclear cells transplantation into the heart could promote angiogenesis and improve heart function.
出处
《中国介入心脏病学杂志》
2004年第5期293-296,共4页
Chinese Journal of Interventional Cardiology
