摘要
目的 建立反相高效液相色谱法测定复方汉防己颗粒中黄芪甲苷的含量。方法 采用AlltechC18(2 5 0mm× 4 .6mm ,5 μm)色谱柱 ,流动相 :乙腈 -水 (33∶6 7) ,流速 1ml/min ,柱温 :30℃ ,检测器 :ELSD2 0 0 0。 结果 黄芪甲苷在 0 .976～ 4 .88μg范围内线性关系良好 ,r=0 .9997,RSD =2 .1% ,回收率为 98.10 %。结论 本方法简便、灵敏准确、重现性好 ,可用于控制复方汉防己颗粒的质量。
Aim To develop a method using RP-HPLC for determination the content of astragaloside Ⅳ in Fufang Hanfangji Granule.Methods The Alltech C 18 column(250mm×4.6mm,5μm) was used.The mobile phase was composed of acetonitrile-water (33∶67).The flow rate was 1ml/min.The column temperature was 30℃.An evaporative light scattering detector(ELSD) Model 2000 was used as detector.Results The linear range of astragaloside Ⅳ was 0.976～4.88μg(r=0.999 7,RSD=2.1%).The average recovery rate of astragaloside Ⅳ was 98.10%,and RSD=1.6%(n=5).Conclusion The method is simple and accurate and can be used reliably for the quality control of astragaloside Ⅳ in the compound Hanfangji Granule.
出处
《解放军药学学报》
CAS
2004年第5期350-352,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
