摘要
Objective: To analyze the association between β2-adrenergic receptor (β2-AR)genetic polymorphisms and nocturnal asthmatic patients of Chinese Han nationality. Methods: Polymerase chain reaction-allele specific oligonucleotide hybridization assay was used to determine 16 and 27 locus alleles of β2-AR genetic polymorphisms in 25 nocturnal asthmatic patients( NA group). 22 nonnocturnal asthmatic patients (NNA group) and 72 healthy people ( control group). All subjects were from Chinese Han nationality. Results. The distribution frequency of genotype Arg/Arg, Arg/Gly, and Gly/Gly at β2-AR 16 loci was 12%, 16% and 72% in NA group; and was 27%, 41% and 32% in NNA group. There was significant increase in the frequency of genotype Gly/Gly and allele Gly in NA group, compared with NNA group(P<0.01). However, there was no significant difference in the frequency of genotype Gly/Gly and allele Gly in NNA group, compared with control group. No significance in the frequency of the genotypes and alleles of β2-AR27 loci among the three groups(P>0.05). Conclusion: The Gly16 polymorphism of β2-AR is overrepresented in nocturnal asthmatic patients, correlated with nocturnal asthma and therefore appears to be an important genetic factor in the expression of this asthmatic phenotype.
