摘要
目的:观察中药肝脂复煎剂对酒精和高脂饲养诱导的大鼠脂肪肝的防治作用. 方法:采用高浓度酒精灌胃合高脂饲料饲养制备大鼠脂肪肝模型,同时给予不同剂量肝脂复煎剂治疗,以二甲双胍为对照.通过检测血清肝酶谱、肝组织匀浆甘油三脂含量和肝脏组织学变化,观察不同剂量肝脂复煎剂对脂肪肝的防治作用. 结果:模型组大鼠肝脏出现明显的脂肪变性,肝酶活性(ALP、GGT)、血脂和肝组织甘油三脂含量升高(ALP: 8 156±2 696 vs 4478±2 229;GGT:52±14 vs 24±21; TG:615±106 vs 454±113,P<0.05;肝TG:53±10 vs 27±8,P<0.01).肝脂复剂量组肝细胞脂肪变显著减轻, 肝酶活性(ALP、GGT)、血脂和肝组织甘油三脂含量较模型组显著下降(ALP:5 666±2 187 vs 8 156±2 696;GGT: 24±14 vs 52±14;TG:442±148 vs 615±106;肝TG: 35±4,36±6,38±6 vs 53±10,P<0.05-P<0.01), 二甲双胍组肝组织甘油三脂含量较模型组下降(32±1 vs 53±10,P<0.01),其余指标无显著差异.
AIM: To explore the effects of Chinese medicine Ganzhifu decoction on fatty liver in rats. METHODS: The fatty liver model was established by feeding with high caloric laboratory chaw and alcohol. Sixty male Wistar rats were randomized into 6 groups, i.e. a normal control group, a model group, three Ganzhifu groups treated with Ganzhifu decoction (giant knotweed 20 g, rhubarb 5 g, Bupleurum 10 g) in different concentrations and a metformin group. All the animals were killed after treatment for 8 weeks. The activities of alkaline phosphatase and gamma glutamyl transferase, blood lipid, liver triglycerin and pathological changes in all groups were assayed. RESULTS: The activities of alkaline phosphatase and gamma glutamyl transferase, blood lipid, liver triglycerin of model group increased significantly (ALP: 8 156±2 696 vs 4 478±2 229; GGT: 52±14 vs 24±21; TG: 615±106 vs 454±113, P<0.05; Liver triglycerin: 53±10 vs 27±8, P<0.01). Liver histology showed mild to moderate steatosis. Those indexes of Ganzhifu groups were significantly lower than those of model group (ALP: 5 666±2 187 vs 8 156±2 696; GGT: 24±14 vs 52±14; TG: 442±148 vs 615±106; Liver triglycerin: 35±4, 36±6, 38±6 vs 53±10, P<0.05, P<0.01). The hepatic steatosis was also improved. Liver triglycerin of metformin group was significantly reduced (32±1 vs 53±10, P<0.01), but there was no significant difference in other indexes between metformin and model groups. CONCLUSION: Ganzhifu decoction can protect fatty liver in rats.
出处
《世界华人消化杂志》
CAS
2004年第7期1596-1599,共4页
World Chinese Journal of Digestology
基金
湖北省重点攻关项目
No.2002AA302B13~~
