摘要
目的 探讨胎盘组织波形蛋白、Ⅳ型胶原蛋白和纤连蛋白的表达与妊娠高血压综合征(妊高征 )发病的关系。方法 采用免疫组化过氧化物酶 抗过氧化物酶 (PAP)法 ,检测 19例妊高征患者 (妊高征组 )胎盘组织波形蛋白、Ⅳ型胶原蛋白和纤连蛋白的表达情况 ,并与同孕周的正常孕妇15例 (对照组 )进行比较。结果 (1)中、重度妊高征患者胎盘组织波形蛋白阳性反应平均灰度值为30± 7,明显低于对照组的 4 2± 6和轻度妊高征患者的 4 2± 9,差异有极显著性 (P <0 0 1) ;(2 )中、重度妊高征患者胎盘组织Ⅳ型胶原蛋白阳性反应平均灰度值为 70± 6 ,较对照组的 2 9± 4和轻度妊高征患者的 30± 5明显增强 ,差异也有极显著性 (P <0 0 0 1) ;(3)中、重度妊高征患者胎盘组织纤连蛋白阳性反应平均灰度值为 37± 4 ,较对照组的 31± 4和轻度妊高征患者的 32± 6增强 ,差异有显著性 (P <0 0 5 )。结论 胎盘组织波形蛋白、Ⅳ型胶原蛋白和纤连蛋白表达异常 ,可能与妊高征的发病有密切关系 。
Objective To elucidate the possible relationship between pregnancy-induced hypertension syndrome (PIH) and expression of vimentin, type Ⅳ collagen and fibronectin in human placenta. Methods Nineteen pregnant women ( PIH group ) and 15 normal pregnant women (control group ) matched for age and gestation were recruited. The expression of vimentin, type Ⅳ collagen and fibronectin in the placenta of PIH patients and normal subjects was investigated using immunohistochemical perioxidase-antiperoxidase (PAP) staining technique combined with an analysis through a computer-aided microphotographic system and compared between the two groups. Results The average grey value difference (GVD) which indicated positive staining of vimentin was 30±7 in section images of the moderate and severe PIH group, significantly lower than that of normal control group, 42±6 and mild PIH group, 42±9 (P<0.01). The average GVD of type Ⅳ collagen in moderate and severe PIH group was 70±6, significantly higher than 29±4 of control group and 30±5 of mild PIH group (P<0.001).The average GVD of fibronectin in moderate and severe PIH group was 37±4 , significantly higher than that in control group, 31±4 and mild PIH group, 32±6 (P<0. 05). Conclusions There is tight relationship between abnormal expression of placental vimentin, type Ⅳ collagen, fibronectin and pathogenesis of PIH. Furthermore, the severity of PIH is positively correlated with the abnormal expression levels of those proteins.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2004年第9期609-611,i001,共4页
Chinese Journal of Obstetrics and Gynecology
