摘要
目的 探讨CD3 6在多囊卵巢 (PCO)发病中的局部分子机理。方法 选择因不同指征行腹腔镜手术的典型多囊卵综合征 (PCOS)患者 8例 (PCOS组 ,双侧卵巢标本共 16份 ) ,不对称性PCO患者 12例 (PCO组 ,PCO标本 12份 ,正常卵巢标本 8份 ,其余卵巢未分离出卵泡 ) ,非内分泌性原发或继发不孕患者 15例 (对照组 ,卵巢标本 19份 ,其余卵巢未分离出卵泡 )。提取卵泡液 ,测定卵泡液中睾酮、硫酸脱氢表雄酮 (DHEAS)、雄烯二酮 (A2 )和胰岛素水平 ;采用RT PCR半定量方法 ,检测卵泡内膜细胞CD3 6mRNA的表达水平。结果 PCOS组患者卵巢组织中CD3 6mRNA的相对表达量为 0 2 1±0 0 5 ,PCO组患者PCO组织中CD3 6mRNA的相对表达量为 0 2 4± 0 0 7,均明显低于PCO组患者正常侧(0 83± 0 0 8)和对照组卵巢组织 (0 83± 0 13) ,差异有极显著性 (P <0 0 1) ;PCO卵泡液中睾酮、胰岛素水平较正常卵巢组织明显升高 ,差异有极显著性 (P <0 0 1) ;卵巢局部睾酮、胰岛素水平与卵泡内膜细胞CD3 6mRNA的表达水平呈负相关 (P <0 0 1)。结论 CD3 6在转录水平的下降 ,可能通过卵巢局部激素 (睾酮、胰岛素 )水平的变化 。
Objective To detect CD_(36) expressions in polycystic ovary (PCO), and to explore its correlation with local androgen and insulin at transcription level. Methods From August 2002 to February 2003, 12 patients with asymmetric PCO, 15 primary or secondary infertile patients without endocrine disorders and 8 polycystic ovary syndrome (PCOS) with bilateral PCO were recruited. Extraction of follicular fluid and detection of testosterone(T), dehydroepiandrosterone sulfate (DHEAS), insulin (INS) and androstenedione (A_2) were performed. Relative CD_(36) mRNA expression level of human ovarian inner thecal cells was analyzed by auto image analysis system (IAS) after RT-PCR. Results The level of CD_(36) mRNA expression in thecal cells was 0.24±0.07 in polycystic ovary of PCO group and 0.21±0.05 in bilateral ovaries of PCOS group, respectively, which were significantly lower than 0.83±0.13 in normal ovaries (P<0.01). T and INS levels of follicle fluid in PCO were significantly higher than that in normal ovaries (P<0.01). T and INS levels of follicle fluid were negatively related to CD_(36) mRNA expression of follicular theca interna (r=-0.6810, r=-0.6708,P<0.01). Conclusion Decrease of scavenger receptor gene CD_(36) mRNA may play a role in the pathogenesis of PCO by increasing the level of T and INS in follicular fluid.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2004年第9期591-594,共4页
Chinese Journal of Obstetrics and Gynecology
