摘要
目的 探讨苦参碱对大鼠肾小球硬化的早期防护作用。方法 用单侧肾切除加 1周后尾静脉注射阿霉素 (5mg/kg)的方法建立肾小球硬化大鼠模型 ,分模型对照组、苯那普利治疗组、苦参碱 10 0mg/kg治疗组和苦参碱 5 0mg/kg治疗组 ,设假手术组为正常对照组。检测各组术后第 2、4、6周的尿蛋白及第 6周的血清尿素氮、肌酐、总蛋白、白蛋白及肾组织病理改变 ,并应用免疫组织化学方法检测肾组织内纤维连接蛋白 (FN)、层黏蛋白 (LN)在肾小球的沉积和转化生长因子 β1(TGF β1)、结缔组织生长因子 (CTGF)蛋白质的表达。结果 苦参碱 5 0mg/kg治疗组、苦参碱 10 0mg/kg治疗组和苯那普利治疗组的尿蛋白排泄量、血肌酐和尿素氮水平均低于模型对照组 ,肾小球增生、硬化程度轻于模型对照组 (P <0 0 5 ) ,肾小球内FN和LN沉积、肾皮质TGF β1蛋白表达也轻于模型对照组(P <0 0 5 ) ,CTGF仅模型对照组有少量表达 ,正常对照组和各治疗组均无表达 (P <0 0 1)。苯那普利治疗组、苦参碱 10 0mg/kg治疗组以上作用均优于苦参碱 5 0mg/kg治疗组 (P <0 0 5 ) ,但二者间差异无显著意义 (P >0 0 5 )。结论 苦参碱对肾小球硬化大鼠模型肾脏病变有部分防护作用。
Objective Matrine has an anti -fibrosis effect, such as hepatic cirrhosis and derma fibrosis, while its effec t on glomerulosclerosis is unknown. The purpose of this study was to analyze th e renoprotective effects of matrine on experimental glomerulosclerosis in rats a nd inquire into its mechanisms. Methods The rats were ra ndomly assigned to following groups: normal control group, model control group, benazepril treatment group, matrine 100 mg/kg treatment group and matrine 50 mg/ kg treatment group. The rats of normal control group were subjected to sham ope ration and were injected with normal saline via the tail vein one week later. T he rats of the other groups were uninephrectomized and injected with adriamycin ( 5 mg/kg) via the tail vein one week later. The dose of benazepril was 6 mg /kg. Both matrine and benazepril were given by gastric perfusion from the first day after the operation. The level of urinary protein was measured at the 2nd, 4th and 6th week after the operation. The serum total protein and albumin, ser um creatinine, blood urea nitrogen (BUN) were tested only at the 6th week after operation. Renal pathology changes were evaluated at the 6th week as well. Im munohistochemistry was used to detect the expression of fibronectin (FN), lamini n (LN), connective tissue growth factor (CTGF) and transforming growth factor- β1 (TGF-β1) in glomeruli. Results Matrine and benazep ril not only reduced the excretion of urinary protein and the level of serum cre atinine and BUN, but also significantly ameliorated glomerular mesangial prolife ration and glomerular sclerosis (P<0.05, respectively). Immunohistochemica l staining indicated that there was an increasing FN, LN, CTGF and TGF-β1 expr ession in model control group as compared to the three treatment groups (P<0 .05). Matrine 100 mg/kg treatment group and benazepril treatment group showed much more advantages than matrine 50 mg/kg treatment group (P<0.05), but there was no significant difference between the former two groups (P>0
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2004年第10期737-740,共4页
Chinese Journal of Pediatrics
基金
甘肃省中青年科技研究基金 (YS 0 11 A2 3 0 2 2 )
