摘要
目的 :探讨以叶酸介导的MR对比剂Gd DTPA Folate对裸鼠肿瘤信号改变的规律 ,评价其对肝肿瘤诊断的可行性及有效性。方法 :将DTPA Folate与GdCl3 在一定条件下等摩尔反应 ,过滤后得到Gd DTPA Folate ;将 5～ 6周龄SPF级BALB/C裸鼠皮下接种人肝癌细胞 (SMMC 772 1)作为动物模型 ;将裸小鼠随机分成实验组与对照组 ,前者注射Gd DTPA Folate ,后者注射含相同Gd离子浓度的Gd DTPA ,注射部位为尾静脉 ,注射剂量 0 .2ml。观察注射前后两组T1WI瘤体信号改变情况 ,判断指标为对比信噪比 (CNR)。注射后扫描时间点选为即刻、1、2、3、4、6、12及 2 4h。结果 :注射对比剂后 2～ 3h ,实验组裸鼠瘤体信号强度较对照组升高明显 ,差异有统计学意义 (t =4.2 694/2 .2 977,P <0 .0 5 )。结论 :Gd DTPA Folate对裸鼠肝癌模型 (SMMC 772 1)具有一定的靶向性。
Objective:To study the possibility and effectiveness of evaluating hepatic tumor with Gd-DTPA-folate by observing changes of signal intensities of the human hepatoma (SMMC-7721) transplanted in the nude mice.Methods: DTPA-folate and GdCl3 were first conjugated under certain conditions.Then,Gd-DTPA-folate became available after filtering;nude mice transplanted with humah hepatoma (SMMC-7721) were used as animal models,they were divided into experimental group and control group respectively.The former group was injected with Gd-DTPA-folate,the later was injected with Gd-DTPA(containing same concentration of gadolinium).Contrrast agent was injected via tail vein.Tumor signal of experimental group was compared with that of control group.CNR (contrast-noise-ratio) was regarded as standard for evaluation.Results:Signal intensity of tumor in experimental group enhanced evidently between 2～3h after injection contrast agent,compared with that of control group.Conclusion:Gd-DTPA-folate presents certain characteristic of tumor targerting of nude mice transplanted with human hepatoma (SMMC-7721).
出处
《放射学实践》
2004年第11期779-782,共4页
Radiologic Practice
基金
国家自然科学基金资助项目 (39770 2 340 )
关键词
受体
叶酸
对比剂
磁共振成像
肝肿瘤
小鼠
裸
Receptors,folic acid
Contrast agent
Magnetic resonance imaging
Liver neoplasms
Mice,nude
