摘要
将本室克隆的编码复制酶基因3′端约1/2序列及其3′端非编码区的苜蓿花叶病毒中国分离株(AlMV-Ch)RNA23′端cDNA,重组到植物表达载体pROKII中,通过致瘤农杆菌(A-grobacteriumtumefaciens)介导,以叶圆片为转化材料,转化普通烟草,并获得了转基因植株.经卡那霉素抗性选择、PCR检测目的基因证明AlMVRNA23′端基因已整合到转基因烟草的基因组DNA中.转基因植物的攻毒试验表明转基因植株对苜蓿花叶病毒产生高水平的抗性.
The cloned 3′terminal cDNA fragment of Alfalfa Mosaic Virus Chinese isolate(AlMV-Ch )was constructed into plant transformation binary vector pROKII, then introduced into the ordinary tobacco (Nicotiana tabacum) cultivar via Agrobacterium tumefaciens.The PCR detection of transgenic tobacco plants showed that the 3′terminal cDNA fragment of AlMV-Ch RNA_2 had been integrated into the genome of tobacco plants.The virus-inoculation test showed that the transgenic lines produced a high level resistance to AlMV infection.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第6期663-667,共5页
Journal of Inner Mongolia University：Natural Science Edition
基金
国家自然科学基金资助项目(39860042)
关键词
苜蓿花叶病毒
复制酶基因
抗病毒
Alfalfa Mosaic Virus (AlMV)
replicase gene
anti-virus
