摘要
目的 用共表达转化生长因子 β(TGF β)和实验性变态反应性脑脊髓炎 (EAE)自身抗原表位蛋白脂蛋白 (PLP)的重组腺病毒载体转染小鼠树突状细胞 (DC) ,检测转基因DC的表型特征和功能特点。方法 构建共表达TGF β和EAE自身抗原表位的重组腺病毒载体转染小鼠骨髓造血细胞培养的DC。检测重组腺病毒的转染效率、转基因DC对抗脂多糖 (LPS)促成熟的能力、介导同种异体T细胞增殖能力、抗原递呈功能的改变、吞噬功能分析及表面表达自身抗原的情况。结果 该双表达重组腺病毒能高效转染DC并得到了正确表达 ,转基因DC比对照病毒载体更能对抗LPS的促成熟作用 ,它刺激同种T细胞增殖能力和抗原特异性增殖反应均受到一定程度的抑制 ,其抗原内吞能力较对照病毒载体增高。转基因DC在表达TGF β的同时 ,表达了自身致病抗原表位。结论 TGF β和PLP/Ig DC双基因修饰的DC可使DC维持在相对非成熟状态 ,在体外诱导T细胞抗原特异性低反应性。
Objective To observe the change of surface marker and function in bone marrow derived dendritic cell(DC) transduced by mouse TGF-β and autoantigen epitope recombinant adenovirus vector. MethodsTGF-β and autoantigen epitope recombinant adenovirus vector was constructed to transduce BMDC. The transduction efficiency, the capability of transgenic DC to counter lipopolysaccharide(LPS), to induce allogenic T cells proliferation, to present antigen, and to intake antigen were detected and autoantigen epitope expression in transgenic DC surface was analyzed. Results The adenovirus were constructed could transduce DC efficiently and expressed transgene correctly, the transgenic DC was shown to counter the maturation effect of LPS in compared with LacZ control adenovirus, its capability to stimulate allogenic T cells and specific antigen proliferation was inhibited in some extent as compared to control adenovirus. Transgenic DC could express autoantigen epitope in surface in addition to the TGF expression. Conclusion The transgenic DC expressing TGF-β and autoantigen epitope were in a relative immature state and could induce antigen specific hyporesponse in vitro.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2004年第10期759-764,共6页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金资助项目 (NO .3 9970 2 70 )
