摘要
目的研究不同剂量和不同给药次数的链脲霉素(STZ)诱发胰岛炎和损害α、β细胞的程度。方法将Wistar大鼠分成6组对照组;A组STZ50mg·kg-1·d-11次给药;B组STZ50mg·kg-1·d-连续2d给药;C组STZ50mg·kg-1·d-1连续3d给药;D组STZ30mg·kg-1·d-1连续5d给药;E组STZ80mg·kg-1·d-11次给药。观察各组大鼠的体重和末梢血糖变化,择期摘取大鼠的胰腺制作成组织切片,进行HE,Azan和免疫组织化学染色,并在光学显微镜下进行组织学比较。结果与对照组相比,各组大鼠的胰腺均存在明显的胰岛炎,D组胰岛的炎细胞浸润、空洞形成和组织纤维化较其他各组显著严重,各组残留胰岛数量、胰岛平均面积、β细胞面积和β细胞面积胰岛平均面积之比均显著减少,尤其是C、D和E组最明显;相反,各组的α细胞面积和α细胞面积胰岛平均面积之比因α细胞的显露而显著增大。结论STZ可同时诱发化学性胰岛炎和自身免疫性胰岛炎,破坏α细胞和β细胞,使胰岛出现空洞形成和组织纤维化。STZ80mg·kg-1·d-11次给药可造成最严重的胰岛损害。
AIM: To investigate the damage of alpha and beta cells on islets of diabetic rats in different dosages and injecting times of streptozotocin (STZ). METHODS: Wistar rats were divided into six groups: control group, group A (single injection with 50 mg·kg -1 STZ), group B (injection with 50 mg·kg -1 ·d -1 STZ for two days), group C (injection with 50 mg·kg -1 ·d -1 STZ for three days), group D (injection with 30 mg·kg -1 ·d -1 STZ for five days) and group E (single injection with 80 mg·kg -1 STZ). The body weights and concentrations of peripheral glucose of rats were measured regularly. Then the pancreases of rats were selected to make tissue slices. The tissue slices embedded in paraffin were histological observes under the light microscope after staining with Hematoxlin and Eosin (HE), Azan and immunohistochemical techniques. RESULTS: Insulitis was observed in each group throughout the term of experiment. It was more serious in all rats that were injected with STZ than those in the control group. The inflammation, vacuolization and fibrosis of islets in the group D were more serious than those in the other groups. The amount of islets, the average area of islets, the area of beta cells, the ratio of the area of beta cells and the average area of islets in each group were significantly decreased, especially in the group C, D and E. Conversely, the area of alpha cells, the ratio of the area of alpha cells and the average area of islets in each group significantly increased as alpha cells appeared. CONCLUSION: STZ can induce chemical insulitis and autoimmune insulitis, destroy both alpha and deltas cells, and make vacuolization and fibrosis in islets. Single, high dose of STZ can cause the greatest damage.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2004年第10期1128-1133,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
