摘要
目的 研究 5个C1q结合十二肽及其牛血清白蛋白 (BSA)偶联物对C1q的结合活性和溶血活性的影响。方法 人工合成十二肽 ,将其与BSA偶联 ,分别用U937细胞配体结合抑制试验、多聚IgG(AIgG)竞争抑制试验、CH5 0抑制试验测试其生物学活性。结果 游离的线性肽对C1q的结合活性和溶血活性几乎无影响 ,而偶联肽对C1q与U937细胞表面受体、AIgG的结合以及C1q介导的溶血均有抑制作用。结论 本实验获得 5个C1q受体模拟肽 ,它们能抑制C1q与免疫复合物结合 ,从而阻断补体经典途径的激活 。
Objective To study the effects of five C1q-binding 12-mer peptides and the conjugates of them to bovine serum albumin (BSA) on the binding and hemolytic activities of C1q. Methods Five 12-mer peptides were synthesized and conjugated to the BSA. The synthetic peptides and the conjugates were tested with the U937 C1q receptors (C1qR), aggregated immunoglobulin G (AIgG), and the C1q-me- diated hemolysis. Results The synthetic peptides had no effects on the activities of C1q, but the synthetic peptide-BSA conjugates strongly inhibited the C1q-mediated hemolysis, and the binding of C1q to C1qR and AIgG in vitro. Conclusion The five mimic peptides of C1qR may inhibit the binding of C1q to immune complex and block the activation of the classical complement pathway, which suggested that they have potential value for the prevention and cure of the diseases related to abnormal activation of the complement system.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2004年第6期424-427,共4页
Immunological Journal
基金
国家自然科学基金 (39970 687)
广东省自然科学基金(0 1 0 60 0 )资助项目
关键词
C1Q
合成肽
抑制
C1q
Synthetic Peptides
Inhibition
