摘要
目的:建立高效液相色谱/质谱联用法测定人血浆中替米沙坦的浓度。方法:液相色谱:采用Agilent Zorbax C18色谱柱(100 mm×2.1 mm,3μm);流动相为甲醇-水(70:30),流速:0.2 mL·min～1,进样量10μL;质谱:大气压化学电离源(AP-CI),选择性检测质荷比(m/z)为513(替米沙坦)和434(内标)带负电荷的分子离子峰定量。样品用固相萃取小柱提取处理。结果:本法线性范围为1.0～500μg·L～1,最小检出浓度为1.0μg·L-1(S/N≥5)。血浆中替米沙坦的回收率为99.3%-106.5%,日内RSD≤10.4%,日间RSD≤14.0%。结论:本法适用于临床替米沙坦治疗药物监测。
Objective: To develop an HPLC/MS method for the determination telmisartan in human plasma. Methods :LC:Use Agilent zorbax C18column(100 mm ×2. 1 mm,3 μm) ;The mobile phase consisting of methanol -water (70:30) ,a flow rate of 0. 2 mL · min-1,injection volume 20 μL. MS;Use atmospheric pressure chemical ioniza-tion(APCI) ,and selected ion mass spectral ( m/z) 513 (telmisartan) and 434 (internal standard) to quantify. The plasma samples were extracted with solid phase extraction. Results;The linear range of telmisartan was 1. 0 -500 μg · L-1 and the limit of detection was 1.0 μg · L-1 (S/N≥5). The recoveries of telmisartan were 99.3% -106. 5%. RSD of intra - day and inter - day were less than 10. 4% and 14. 0% , respectively. Conclusion: The method appears to be appropriate for the therapeutic monitoring of patients.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第5期497-499,共3页
Chinese Journal of Pharmaceutical Analysis
