摘要
目的:测定经拆分合成的左旋S-(-)-甲磺酸罗哌卡因中右旋R-(+)-甲磺酸罗哌卡因含量。方法:采用高效液相色谱法测定。色谱条件:Chiral—AGP柱(150 mm×4.0 mm,5 μm),流动相为异丙醇-磷酸盐缓冲液[取1 mol·L-1的磷酸二氢钠7.5 mL,0.5 mol·L-1的磷酸氢二钠溶液28.5 mL,加水稀释到1000 mL,调节pH值至7.2](7:93),柱温:25℃,流速为1.0 mL·min～1。紫外检测器,检测波长220 nm,进样量20μL。结果:甲磺酸罗哌卡因消旋体中左旋体、右旋体的保留时间分别为12.1及17.4 min,左、右旋体的分离度良好。精密度试验的RSD为3.3%。结论:采用本方法可简单、准确地测定甲磺酸罗哌卡因中右旋甲磺酸罗哌卡因的含量。
Objective: To study a method for the determination of R - ( + ) - ropivacaine mesylate in S - ( - ) -ropivacaine mesylate by HPLC. Method; Chiral -AGP column (150 mm×4. 0 mm, 5 μm) was used as the stationary phase,the mobile phase was isopropanol and phosphate buffer(1 mol · L-1 NaH2PO4,7. 5 mL,0. 5 mol · L-1 Na2 HPO4,28. 5 mL, adjusted pH to 7. 2 with H3 PO4) (7:93). The flow rate was 1. 0 mL · min -1, the column temperature of 25 ℃ and detection wavelength was 220 nm. Results;The R-(+)-ropivacaine mesylate was satisfactorily separated from 5 - ( - ) - ropivacaine mesylate. Conclusion; The method is simple and accurate.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第5期483-484,共2页
Chinese Journal of Pharmaceutical Analysis
