摘要
目的 研究电针刺和尼卡地平对缺血 /再灌注后心肌细胞Hsp70mRNA表达和体内多巴胺含量的影响 ,以及电针刺和尼卡地平之间的相互作用。方法 2 4只兔子随机分为 4组 :对照组 (缺血 /再灌注组 ) ,电针刺组 ,尼卡地平组 ,电针刺 +尼卡地平组 ,每组各 6只。采用夹闭心脏冠状动脉前降支 30min后松解 2h制成缺血 /再灌注模型。术中分别采取电针刺激和静脉给予尼卡地平的处理。在缺血前、缺血 30min和再灌注 2h分别取静脉血测定血中多巴胺的含量 ;取缺血区和非缺血区心肌通过PT -PCR方法测定Hsp70mRNA的表达 ,观察电针刺和尼卡地平对Hsp70mRNA表达及血中多巴胺含量的影响。结果 电针刺组和尼卡地平组的Hsp70mRNA的表达和对照组相比均有明显的增加 (P <0 0 5 ) ,而血中多巴胺含量和对照组相比有明显的降低 (P <0 0 5 ) ,并且和电针刺组、尼卡地平组相比 ,电针刺复合尼卡地平组的变化更为显著 (P <0 0 5 )。结论 电针刺和尼卡地平对缺血 /再灌注后Hsp70mRNA的表达有显著的增强作用 ,能抑制缺血 /再灌注后体内多巴胺含量的增加 。
Objective To study the relationship of Electroacupuncture(EA) and Nicardipine on Hsp70 mRNA expression and the content of Dopamine in blood;and the relationship between EA and Nicadipine.Methods 24 rabbits were divided into 4 groups randomly:control group,EA group,Nicardipine group,EA+Nicardipine group.The ischemia-reperfusion model was made by clipping descending branch of coronary artery then loosing it for 2 hours.EA and Nicardipine were accepted in different groups operation.The content of Dopamine was estimated from blood before ischemic,30 minutes′ ischemic and 2 hours′ reperfusion.Ischemic and non-ischemic myocardium tissues were cut to observe the expression of Hsp70 mRNA by RT-PCR method.Results In EA group and Nicardipine group the expression of Hsp70 mRNA was higher than control group( P <0 05),the content of Dopamine decreased in EA group and Nicardipine group compared with control group( P <0 05);and the changes were more obvious in EA+Nicardipine group than in EA group and Nicardipine group ( P <0 05).Conclusions Electroacupuncture and Nicardipine have Hsp70 mRNA expressing raise and depress the increase of Dopamine in blood after ischemia,and there are synergistic actions between electroacupuncture and Nicardipine.[
出处
《中国急救医学》
CAS
CSCD
北大核心
2004年第7期496-498,共3页
Chinese Journal of Critical Care Medicine
基金
国家自然科学基金项目 (No.3 9970 92 1)
上海市卫生局重大课题 (No .2 0 0 0 -3 )
