摘要
目的 通过对散发性大肠癌中错配修复基因表达的检测,探讨错配修复基因与大肠癌发生和演进的关系。方法 采用蛋白提取技术提取肿瘤组织蛋白,利用免疫印记技术对hMSH2和hMLH1蛋白表达情况进行检测,应用方差分析和t检验对数据进行统计学分析。结果 70岁以上组hMLH1蛋白表达水平显著低于70岁以下两组,在不同分化各组间差异有显著性;右半结肠癌hMHL1蛋白表达显著低于除直肠以外其他部位的肿瘤;无转移肿瘤hMLH1蛋白的表达显著低于有转移肿瘤;其表达与性别无显著相关。hMSH2蛋白表达水平与患者性别、年龄、肿瘤部位、分化程度及转移间均无显著相关。结论 高龄所造成的hMLH1基因蛋白表达降低可能是导致散发性大肠癌的相关因素之一;hMLH1基因表达降低与右半结肠癌的发生关系密切并影响其分化;hMLH1基因表达较高的肿瘤可能易出现转移。hMSH2基因与大肠癌临床病理间无密切联系。
Objective To explore the relationship between the protein expression of mismatched repair gene hMSH2,hMLH1 and the carcinogenesis as well as evolution of sporadic colorectal cancer through examination of expression of the mismatched repair gene in the sporadic colorectal cancer. Methods The protein of cancer tissue was extracted by protein extraction technology. Immunoblotting was used to test the protein expression of hMSH2 and hMLH1. One way analysis of variance and t test were used for statistical analysis. Results The level of hM-LH1 expression in 70 and over 70 years old group was significantly lower than that in under 70 years old groups ( P
< 0.05) .The expression in group with metastasis was significantly lower than that in group without metastasis ( P
< 0.05). The expression in ascending colon cancers was significantly lower than that in the cancers of other locations except rectum (P < 0. 05) . There was significant difference between any two differentiation groups ( P < 0.05) . The expression level of hMSH2 was not significantly related to sex, age, locations of cancer, differentiation and metastasis (P > 0.05). Conclusions Reduction of the expression of hMLH1 with advanced age may be one of the mechanisms causing colorectal cancer. Low expression of hMLH1 is associated closely with the colorectal cancer originating from ascending colon and the differentiation. The cancers with high expression of hMLH1 are more likely to metastasize.The expression of hMSH2 is not closely related to clinical pathological profile of colorectal cancer.
出处
《中国肿瘤临床与康复》
2004年第5期395-397,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
基金
中国科学院知识创新工程领域前沿基金项目(N0.DICPkaaaabc)
