摘要
本文报道用反相高效液相色谱法测定人血清及尿中环丙氟哌酸浓度。血清或稀释尿样加入磷酸盐缓冲液(pH7.5)和十二烷基硫酸钠溶液后,用5ml氯仿萃取。采用Ultrasphere-ODS分析柱,以0.02mol/L醋酸盐缓冲液(pH3.0):乙腈:二甲基甲酰胺:10％四丁基氢氧化铵水溶液=88:6.5:5:0.5为流动相,依诺沙星作内标,在276nm波长下监测洗提液,按内标法定量。本法具有快速简便,灵敏准确等特点,适用于环丙氟哌酸血药、尿血浓度测定及药代动力学研究用。
A sensitive and rapid me-thod for the determination of ciprofloxa-cin using enoxacin as the internal stan-dard was reported. High-performance li-quid chromatograph model 344 (Beckman,USA) with a variable wavelength UVdetector and reversed-phase Ultrasphere-ODS column (5μm, 250×4.6mm) was usedSerum or urine sample preparation invol-ved addition phosphate buffer (pH7.5)and aqueous solution of sodium laurylsulfate, followed by chloroform extrac-tion. The organic layer was removed andevaporated to dryness under an air streamin a 37℃ water bath. The residue wasdissolved in 50μl mobile phase and 20μlinjected. The mobile phase of 0.02 mol/Lacetate buffer (pH 3.0)-acetonitril-dime-thy1formamide-10% aqueous solution oftetrabutyl ammonium hydroxide (88: 6.5:5:0.5) was pumped at 0.9ml/min throughthe column. The detector operated at 0.01aufs and the wavelength was set at 276nm. The retention times for ciprofloxacinand enoxacin were 7.31 min and 5.59 min,respectively. In serum, standard curve was linearin the concentration range of 0.75 to 24μmol/L, the datective limit was 0.2μmol/L, extraction recovery was 69-74%,within-day CV was less than 5%, andinter-day CV was less than 6%.
出处
《华西医科大学学报》
CAS
CSCD
1993年第1期104-107,共4页
Journal of West China University of Medical Sciences
关键词
环丙氟哌酸
高效液相色谱
Ciprofloxacin
Biological fluid sample
Reversed-phase HPLC
