摘要
作者以质粒pUC19为载体,从重组cos质粒pCM1015中分离编码人巨细胞病毒(HCMV)150kd磷蛋白(pp150)主要DNA片段—EcoRI—Y片段,构建了该片段的克隆,依据插入方向不同分别为重组质粒pHCY1和pHCY2,经限制性内切酶酶切分析及Southern印迹杂交鉴定,克隆构建成功,插入片段大小无改变。采用微机系统分析pp150抗原决定簇DNA编码区段的限制性酶切位点,表明该编码区段具有76种限制性内切酶位点,酶切片段大小在4bp～2667bp之间。
Human cytomegalovirus(HCMV) DNA EcoRI-Y fragment, whichis major DNA fragment coding for 150kdphosphoprotein(pp150)of HCMV, was sepa-rated and recovered from recombinantcosmid pCM1015. The recombinant plas-mids pHCY1 and pHCY2, which containmajor DNA fragment coding for pp150,were constructed successfully,using theplasmid pUC19 as vector.The recombinantplasmids were analysed by restriction en-donucleases and determined by Southernhybridization.The restriction sites of DNAencoding region for immunodominant epi-topes of HCMV pp150 were analysed byGENEPRO system. The region could bedigested by 76 kinds of restriction endo-nucleases.The sizes of cleaved fragmentswere between 4-2667bp.
出处
《华西医科大学学报》
CSCD
1993年第1期5-8,共4页
Journal of West China University of Medical Sciences
基金
国家自然科学基金青年基金~~
关键词
巨细胞病毒
磷蛋白
DNA
分子克隆
Human cytomegalovirus
Phosphoprotein
Molecular cloning
Restriction site
