摘要
目的 :研究江浙蝮蛇蛇毒蛋白诱导K5 6 2细胞调亡。方法 :通过电镜观察蛇毒蛋白作用后K5 6 2细胞的形态变化 ;MTT检测蛇毒蛋白对细胞增值的影响 ,同时应用流式细胞仪检测细胞凋亡数及其对细胞周期的影响 ;采用琼脂糖凝胶电泳观测凋亡片断。结果 :蛇毒蛋白作用K5 6 2细胞后 ,能显著抑制细胞增值 ;LC5 0为4 .96 μg/mL ,电镜可观察到凋亡形态学改变 ;电泳呈现典型的阶梯状条带 ,流式细胞仪检测到凋亡峰。 结论 :江浙蝮蛇蛇毒蛋白可诱导K5 6 2细胞调亡。
Aim:To study the protein purified from Agkistrodom halys Pallas venom might induce apoptosis in K562 cells.Methods:Inhibitions of proliferation was measured using MTT assay.The amount of apoptotic cells was measured by flow cytometry.DNA fragmentation was visualized by DNA agarose gel electrophoresis,and the cellular changes were observed with electron microscope.Results:The protein purified from Agkistrodom halys Pallas venom elicited typical apoptosis morphological changes and DNA fragmentation in a concentration-dependent manner in K562 cells.The protein inhibited K562 cells proliferation in concentration-dependent manner,the LC50 is 4.96 μg/mL.Conclusion:The protein purified from Agkistrodom halys Pallas venom induced apoptosis in K562 cells.
出处
《天然产物研究与开发》
CAS
CSCD
2004年第5期387-390,共4页
Natural Product Research and Development
