摘要
以稳定抗生理干旱的愈伤组织为材料,研究了抗旱愈伤组织的抗旱机理.结果表明:抗旱愈伤组织的干物质含量明显高于对照.水势为-10.76bar,而对照愈伤组织的水热为-4.77bar.脯氨酸的含量为对照的4.4倍,K^-和蔗糖的含量比对照稍低.脱落酸的含量是对照的3.4倍,但远远低于对照愈伤组织在含20％聚乙二醇的培养基上逆境四天的脱落酸含量.电泳结果表明:抗性意伤组织中产生了新的蛋白质或多肽(分子量:17000,18000道尔顿),抗性愈伤组织细胞的膜特性得到了改善.提出了抗旱机理假设.
Studies were carried on the mechanism for drought resistance, using stable callus resistant to physiological drought as material. The results showed: The content of dry matter was obviously higher than that of CK. Its water potential was - 10. 76bar, while the water potential of CK was -4. 77bar. Proline content was 4. 4 times as high as that of CK,and the content of k as well as sucrose was slightly lower than that of CK The content of Abscisic Acid was 3. 4 times as high as that of CK.but obviously lower than that of CK stressed on medium containing 20% PEG for four days. New proteins or polypeptides (M. W 17000. 18000 dorton) were produced in the calllus. The property of cell membrane was improved. An assumption of the mechanism for drought resistance was put forward.
出处
《华南农业大学学报》
CAS
CSCD
1993年第1期60-64,共5页
Journal of South China Agricultural University
基金
国家自然科学资金
关键词
苜蓿
抗旱性
愈伤组织
Alfalfa
Resistant Callus
Mechanism for drought resistance
PEG (Polyethylene Glycol) Mw6000
