摘要
目的:探讨回输自动员人外周造血干细胞(PBSC)体外诱导树突状细胞(DCs)治疗乳腺癌患者的可行性。方法:采用CS-3000血细胞分离系统分离9例粒细胞集落刺激因子(G-CSF)预动员乳腺癌患者PBSC,在体外经rhGM-CSF、rhIL-4诱导和自体肿瘤细胞提取物刺激后成为成熟DCs,收集并自体回输。用MTT法测定DCs在体内外激活细胞毒性T细胞(CTL)的体外杀伤活性。结果:9例患者平均采集1.02×109PBSC,培养后平均收获2.3×108DCs,CD83表达率平均为68.7%。收获DCs的数量是等量未动员全血的57.5倍。在体外自体肿瘤抗原致敏的DCs激活CTL对自体原代培养的肿瘤细胞具有显著的杀伤活性。DC瘤苗自体回输除轻微发烧外,无任何其它不良反应,回输后患者PBMC对自体肿瘤细胞的杀伤活性提高20倍。结论:用血细胞分离机分离G-CSF动员肿瘤患者PBSC,在体外诱导制备自体DC瘤苗,回输治疗可提高对自体肿瘤细胞的杀伤活性,而且安全可靠,无明显不良反应。
Objective: To explore the possibility of transfusing dendritic cells(DCs) induced from pre-mobilized human peripheral blood stem cells (PBSC) for therapy of breast cancer patients. Methods: PBSCs were collected from 9 breast cancer patients who were pre-mobilized with G-CSF by CS-3000plus Blood Cell Separating System. After 7-8 days' culture in medium containing rhGM-CSF and rhIL-4 and exposure to autologous cancer cell extracts, they became mature DCs and transfused back to the patients. The killing activity of CTLs activated by mature DCs in vitro and in vivo was determined by using MTT method. Results: The average PBSC number collecting from 9 patients was 1.02×109. After culture, average 2.3×108 DCs were harvested averagely. The CD83 positive accounted for 68.7% of DCs. DCs harvested by this method was 57.5 folds of the equal volume nonmobilized whole blood. CTL induced by mature DCs killed the cultured autologous primary cancer cells powerfully. After DCs transfusion, the patients only felt slight fever and no other side effects, the cytotoxicity of PBMC increased 20 fold than before. Conclusions: PBSC were collected from cancer patients pre-mobilized with G-CSF using blood cell separating system and then cultured to induce mature DCs. DCs transfusion could increase the specific killing activity to autologous cancer cells. No severe side effects were observed.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2004年第20期1150-1152,共3页
Chinese Journal of Clinical Oncology
基金
山东省卫生厅科研基金资助(编号:1999CAIDABBL)
关键词
造血干细胞
树突状细胞
乳腺癌
细胞毒性T细胞
Peripheral blood stem cells(PBSC) Dendritic cells(DCs) Breast cancer
Cytoxic T lymphocytes(CTL)
