摘要
背景:阿尔茨海默病(Alzheimerdisease,AD)发病过程中炎症反应的持续存在是否与病情加重呈正相关性。目的:研究补体受体2型(CR2)在AD患者海马、颞叶脑组织中的表达特征。设计:抽样调查。地点和对象:实验地点为KinsmenLaboratoryofNeurologicalResearch,UniversityofBritishColumbia。研究组为19例AD患者海马、颞叶脑组织,对照组为1例帕金森病、1例遗传性帕金森病、3例多发性硬化、5例皮克病、4例癌症患者、2例心血管疾病、1例感染性疾病和1例慢性阻塞性肺炎患者的海马、颞叶脑组织;阳性对照为扁桃体和脾脏组织。干预:以单克隆和多克隆补体受体2型抗体为一抗,应用免疫组织化学方法和蛋白印迹分析方法对研究对象进行CR2表达情况的研究。主要观察指标:各组患者脑组织CR2表达情况。结果:脾脏与扁桃体的树突状细胞胞浆能够被CR2抗体强烈染色。CR2的抗体也使部分对照组的一些血管内圆形细胞着色。在19例AD患者海马和颞叶新皮质中,由变性的神经突起围绕淀粉样核心构成的老年斑和大脑神经元胞浆内的神经纤维缠结均被CR2强烈着色。结论:CR2在AD患者脑组织炎症反应中可能发挥有害作用。
BACKGROUND:Whether the constant existence of inflammatory reaction during the onset of Alzheimer disease(AD)is positively correlated with the aggravation of the disease. OBJECTIVE:To investigate the expressional characteristics of complement receptor type 2(CR2)in the hippocampal and temporal isocortex brain tissue in patients with AD. DESIGN:Sampling investigation. SETTING and PARTICIPANTS:Setting:Kinsmen Laboratory of Neurological Research,University of British Columbia.All the patients' diagnosis was confirmed by routine neuropathological examination in the hospital of University of British Columbia.The study group consisted of the hippocampal and temporal isocortex brain tissues of 19 AD patients while the control group consisted of those of one patient with Parkinson disease(PD),one with familial PD,three with multiple sclerosis,five with Pick's disease,four with cancer,two with cardiovascular disease,one with sepsis and one patient with chronic obstructive pulmonary disease.The tonsil and spleen tissues were used as positive controls. INTERVENTIONS:CR2 monoclonal and polyclonal antibody were used as the first antibody,the methods of immunohistochemistry and Western blotting were used in this study. MAIN OUTCOME MEASURES:The expression of CR2 in the brain tissues of patients in each group. RESULTS:The follicular dendritic cells(FDC)in the spleen and tonsil tissues were intensely stained by CR2 antibodies.The antibody to CR2 also stained occasional round cells in the control group.In the hippocampal and temporal isocortex tissues of 19 patients with AD,the senile plaques and neurofibrillar tangles which were formed by degenerative neurites surrounding amyloid core were both stained strongly by CR2. CONCLUSION:CR2 may play a harmful role in the development of inflammation in AD.
出处
《中国临床康复》
CSCD
2004年第28期6212-6214,共3页
Chinese Journal of Clinical Rehabilitation