摘要
背景:维生素B12缺乏会产生神经功能障碍,影响中枢神经系统及全身的代谢,补充维生素B12对预防及延缓阿尔茨海默病(Alzheimerdisease,AD)的发展作用机制尚不清楚。目的:研究AD与血清维生素B12,叶酸及血浆同型半胱氨酸水平之间的关系,探讨导致痴呆的机制。设计:以诊断为依据的非随机对照研究。地点和对象:2002-10/2003-05于华中科技大学同济医学院附属协和医院住院治疗的AD患者30例(AD组),均符合美国神经病学语言障碍和卒中-阿尔茨海默病和相关疾病学会(NINCDS-ADRDA)的很可能AD标准。对照组为同期参加体检的健康正常人30例。方法:采用简易精神状态量表(MMSE)对两组患者进行评分,并用放免分析法测定血清维生素B12及叶酸水平,荧光偏振免疫法(fluorescencepolarizationimmunoassay,FPIA)测定血浆同型半胱氨酸水平。主要观察指标:①AD组及对照组之间维生素B12水平差异。②AD组及对照组之间Hcy的水平差异。③MMSE得分与维生素B12及Hcy之间的相关性。结果:AD组维生素B12水平(217.3±134.2)pmol/L明显低于对照组(313.6±184.7)pmol/L,二者差异有极显著性(t=3.93,P<0.001)。AD组血浆同型半胱氨酸(18.9±6.8)μmol/L高于对照组(9.4±4.1)μmol/L,差异有极显著性意义(t=7.66,P<0.001)。AD组叶酸水平(29.2±12.7)
BACKGROUND:The deficiency of vitamin B12 will lead to neurological disorder and affect the metabolism of central nervous system(CNS) and the whole body.The mechanism of filling up vitamin B12 in preventing and retarding the development of Alzheimer disease(AD) remains uncertain. OBJECTIVE:To explore the correlations of AD with serum vitamin B12,folate and plasma homocysteine(Hcy)and its mechanism of inducing AD. DESIGN:A non randomized control study based on diagnosis. SETTINGS and PARTICIPANTS:Thirty AD inpatients(AD group),admitted from October 2002 to May 2003 to the Affiliated Xiehe Hospital,Tongji Medical College,Huazhong University of Science and Technology,conformed to the probable AD standard of NINCDS ADRDA.Thirty healthy normal persons who took the health examinaltion at the same time period were recruited to control group. INTERVENTIONS:Patients of the two groups were scored with mini mental state examination(MMSE).The concentration of serum vitamin B12 and folate were examined with the radioimmunoassay. Plasma Hcy was measured with fluorescence polarization immunoassay(FPIA). MAIN OUTCOME MEASURES:①differences of vitamin B12 level in the AD group and the control group.②differences of Hcy level in the AD group and the control group.③the correlations of MMSE score with vitamin B12 and Hcy. RESULTS:The level of serum vitamin B12 in the AD group[(217.3 ±134.2) pmol/L] was significantly lower than that of the control group[(313.6±184.7) pmol/L].There was extremely significant difference between them(t=3.93,P< 0.001).The plasma Hcy in the AD group[(18.9±6.8) μmol/L]was significantly higher than that in the normal group[(9.4±4.1) μmol/L].The differences were significant(t=7.66,P< 0.01).The serum folate concentration in the AD group[(29.2±12.7) nmol/L] was lower than that in the control group[(37.2 ±21.2) nmol/L]but without significant differences(P > 0.05).There was positive correlation between serum vitamin B12 and MMSE scores. CONCLUSION:The level of serum vitamin B12 of AD patie
出处
《中国临床康复》
CAS
CSCD
2004年第28期6210-6211,共2页
Chinese Journal of Clinical Rehabilitation
