摘要
〔目的〕探讨蜂胶对诱变剂诱发的基因突变的抑制作用 ,对诱变剂诱发的染色体损伤的保护作用。〔方法〕应用Ames试验分别测试蜂胶对 2 -氨基芴 (2 -AF)、柔毛霉素 (DNR)、叠氮钠 (NaN3 )诱发的突变的抑制作用。应用小鼠骨髓微核试验及小鼠睾丸染色体畸变试验 ,测试蜂胶对诱变剂诱发的染色体损伤的保护作用。〔结果〕Ames试验发现40 0 0～ 12 0 0 0 μg/皿浓度蜂胶对 2 -AF、DNR、NaN3 三种诱变剂诱发的基因突变均产生抑制作用 ,抑制率分别为 5 6.8%、72 .2 %和 2 0 .8% ,且伴有剂量 -反应关系 ;小鼠骨髓微核试验发现 2 2 5mg/kg、675mg/kg 2个蜂胶剂量对环磷酰胺 (CP)引起的微核的发生产生了明显的抑制作用 ,抑制率分别为 2 0 .7%和 2 4.0 %。小鼠睾丸染色体畸变试验显示 ,675mg/kg蜂胶剂量对丝裂霉素C(MMC)引起的细胞染色体畸变有明显的抑制作用 ,抑制率为 3 1.5 %。〔结论〕蜂胶对 3种诱变剂诱发的不同类型的基因突变均有一定的抑制作用 ,对诱变剂引发的染色体损伤有保护作用。
〔Objective〕 To investigate the antagonistic effect of propolis on mutation and chromosome aberration induced by mutagens.〔Methods〕 The antagonistic effect of propolis to mutation induced by 2-aminofluorane (2-AF),daunorubicin (DNR) and sodium azide (NaN 3) was assessed by Ames test.Micronucleus test of bone marrow cells and chromosome aberration test were performed in mice to assess the antagonistic effect of propolis to mutation induced by mutagens.〔Results〕 The dose of 4000～12000 mg/late of the propolis could inhibit the mutation induced by all the 3 mutagens in a dose-effect manner.At the dose of 225～675 mg/kg,propolis could significantly reduce the rate of micronucleus in mice bone marrow cells induced by cyclophosphamide (CP).Propolis at the dose of 675 mg/kg could markedly reduce the rate of chromosome aberration in mice testicle cells induced by mitomycin C.〔Conclusions〕 Propolis can antagonize the gene mutation and chromosome aberration induced by 2AF,DNR and NaN3 in mice.
出处
《中国卫生检验杂志》
CAS
2004年第4期438-440,共3页
Chinese Journal of Health Laboratory Technology
关键词
蜂胶
诱变剂
基因突变
染色体损伤
Propolis
Mutagen
Gene mutation
Chromosome aberration
