摘要
[目的 ] 探讨乙型肝炎患者血清中HBVDNA含量与HBV血清学标志的关系。 [方法 ] 测定不同乙肝血清学标志模式和 2 47份标志物全阴性的临床肝炎病人血清以半套式PCR荧光定量检测系统检测HBVDNA含量。 [结果 ] 在HBsAg、HBeAg阳性的血清中HBVDNA含量最高 ,血清HBeAg和HBVDNA含量密切相关。但在很少数HBeAg阳性血清中未能检出HBVDNA。在另外的乙肝血清标志物模式中 ,包括全阴性和抗HBs阳性 ,也有少数血清标本能检出HBVDNA ,其浓度分布的范围很广。 [结论 ] 少数血清HBsAg、HBeAg阳性的病人并不处于HBV活跃复制的状态。PCR定量检测HBVDNA含量更有助于乙肝的预后和抗病毒疗效的监测。
Objective] To analysis the relationship between serum HBV DNA levels and the patterns of serological marker. [Method] HBV DNA levels in sera with different HBV serological markers and sera without these markers were measured. By a semi-nested PCR fluorescent quantitating system. [Results] On the whole, there were close relativity between serum HBV DNA and HBeAg. The highest HBV DNA levels were presented in sera positive for both HBsAg and HBeAg, but it was notable that undetectable HBV DNA level could also be found in a few of these sera. Various HBV DNA levels in a wide range were detected in some sera with other patterns of HBV serological markers including a few sera with negative for all HBV markers and even positive for anti-HBs. [Conclusion] A few patients positive for both serum HBsAg and HBeAg may not be in the state of active HBV replication and the serum HBV DNA level quantified by PCR is more helpful for prognosing the illness course and monitoring the efficacy of antiviral therapy of hepatitis B.
出处
《上海预防医学》
CAS
2000年第8期362-364,共3页
Shanghai Journal of Preventive Medicine
