摘要
目的 探讨荧光定量PCR检测HBV—DNA的应用价值。 方法 从 2 92 0份用ELISA检测乙肝 5项的体检血清标本中 ,抽取 2 88份HBsAg阳性标本及 10 0份全阴标本 ,进行荧光定量聚合酶链式反应 (FQ—PCR)HBV—DNA定量分析。 结果 经FQ—PCR检测 ,80份HBsAg、HBeAg、HBcAb都阳性的标本 ,HBV—DNA阳性率为 10 0 % ( 80 /80 ) ,平均HBV—DNA拷贝数为 2 2× 10 8cp/ml ;164份HBsAg、HbeAb、HBcAb都阳性的标本 ,HBV—DNA阳性率为79 3 % ( 13 0 /164 ) ,平均HBV—DNA拷贝数为 1 5× 10 6cp/ml;12份HBsAg单项阳性的标本 ,HBV—DNA的阳性率为83 3 % ( 10 /12 ) ,平均HBV—DNA拷贝数为 1 6× 10 5cp/ml;10 0份HBsAg、HBsAb、HBeAg、HBeAb、HBcAb都阴性的标本 ,HBV—DNA的阳性率为 2 % ( 2 /10 0 ) ,平均HBV—DNA拷贝数为 4 5× 10 5cp/ml。 结论 FQ—PCR可以检测HBV的感染和复制情况 ,对准确报告HBV感染 。
Objective To explore the value in application of Fluorescence quantitative PCR in detection of HBV-DNA in physical examination. Method Fluorescence quantitative PCR (FQ-PCR) was used to measure HBV-DNA of 288 HBsAg+ serum samples and 100 normal samples of 2920 serum samples tested by ELISA method in physical examination. Results 80 HBsAg+ / HbeAg+/HbcAb+ samples were all positive result (80/80), with 2.2×10 8 cp/ml of HBV-DNA on average. In 164 HBsAg+ /HBeAb+ /HBcAb+ samples, the average copy number of HBV-DNA was 1.5×10 6 cp/ml with a positive rate of 79.3% (130/164). In 12 HBsAg+ samples, the average was 1.6 ×10 5cp/ml with a positive rate of 83.3%(10/12). 100 normal samples the average was 4.5×10 5cp/ml with a positive of 2%(2/100). Conclusion FQ-PCR was accurate for detection of HBV and can be used for monitoring the actual state of HBV infection and replication.
出处
《中国热带医学》
CAS
2004年第4期529-530,共2页
China Tropical Medicine
