摘要
目的 :探讨建立反流性食管炎大鼠模型的最佳方案。方法 :将 60只 SD大鼠分为 3组 ,用不同的手术方式建立胃十二指肠混合反流模型 ,A组 :全层间断缝合组 ,B组 :全层间断 +浆肌层包埋缝合组 ,C组 :全层间断密集缝合组 ,并对 3组模型术后不同时期的食管标本进行大体和组织学改变的观察。结果 :术后 A、B、C组的死亡率分别为 75 %、70 %和1 0 % ,主要死亡原因为吻合口瘘、梗阻、肺部及腹腔感染 ,C组与 A、B两组相比有显著性差异 ( P<0 .0 0 1 ) ;3组间反流性食管炎的发生率及组织学改变均无显著性差异 ( P>0 .0 5 )。结论 :全层间断密集缝合是此模型最佳的吻合方式 ,成功模型的建立需要注重多方面的综合因素。
Objective: To investigate the best manier in rat model of reflux esophagitis. Methods: Duodengastroesophageal reflux model were made by three kinds of anastomosis in 60 rats. Group A: single layer of interrupted suture,group B: single layer of interrupted suture plus plasm muscular layer embed suture,group C: single layer of concentrated interrupted suture. The esophageal mucosa samples were taken at different time after operation and studied by gross and microcopic observation. Results: The death rate of group A,B,C after operation was 75%,70% , 10% respectively,group C was much lower than group A and B( P <0.001);the incidence of reflux esophagitis and pathological changes among group A,B,C had no significant difference( P >0.05). Conclusion: Single layer of concentrated interrupted suture was the best anastomosis manier in this reflux model,and there are various factors to be considered in making successful model.
出处
《陕西医学杂志》
CAS
北大核心
2004年第8期677-679,共3页
Shaanxi Medical Journal
基金
卫生部临床学科重点项目 ( No.2 0 0 1 2 1 3 0 )
