摘要
目的探讨系统性硬皮病成纤维细胞自身分泌的转化生长因子(TGF)β1是否影响Ⅰ型胶原α1链(COL1A1)mRNA表达。方法采用核酸原位杂交和免疫印迹法分别测定培养的硬皮病和正常人成纤维细胞中COL1A1mRNA的表达和TGF-β1蛋白质的含量。结果硬皮病组成纤维细胞COL1A1mRNA(A值x±s为905.09±20.36)表达较对照组354.17±30.89明显增高。硬皮病组和对照组成纤维细胞的COL1A1mRNA表达均存在异质性,硬皮病组COL1A1mRNA高表达的成纤维细胞数x±s为27.33%±4.63%明显高于对照3.67%±1.20%,而对照组低COL1A1mRNA表达的成纤维细胞数62.33%±3.53%明显高于硬皮病组14.33%±4.91%。硬皮病组内各细胞株间,高、中、低胶原表达的细胞数差异无显著性。硬皮病组和对照组成纤维细胞中TGF-β1蛋白质表达差异无显著性。结论硬皮病成纤维细胞Ⅰ型胶原mRNA表达增多,这种表达增多与成纤维细胞自身合成TGF-β1的水平可能无关。
Objective To investigate the relationship between the expressions of transforming growth factorβ1(TGF-β1)andα1(Ⅰ)procollagen(COL1A1)in dermal fibroblasts from the patients with sys-temic sclerosis(SSc).Methods The expression of COL1A1mRNA was assessed by in situ hybridization.Western Blotting was used to detect the production of TGF-β1in fibroblasts from either SSc patients or normal controls.Results The mRNA level of COL1A1was higher in fibroblasts from SSc patients(total optical density was905.09±20.36)than that from normal controls(354.17±30.89).Meanwhile,there was heterogeneity of COL1A1mRNA expression in the fibroblasts from both SSc group and normal group.There were more high-COL1A1-expressing fibroblasts in SSc group(27.33%±4.63%),compared with that in control group(3.67%±1.20%).On the other hand,there were less low-COL1A1mRNA-expressing fibrob-lasts in SSc group(14.33%±4.91%)than that in control group(62.33%±3.53%).In SSc patients,the proportion of low,middle and high COL1A1mRNA expressing fibroblasts was similar.TGF-β1production in SSc fibroblasts was similar to that in normal controls.Conclusion The typeⅠcollagen mRNA expression was elevated in SSc fibroblasts,which may be uncorrelated with the TGF-β1.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2003年第9期490-492,共3页
Chinese Journal of Dermatology
