摘要
从受污环境中采集污泥样品,分离筛选出一株对偶氮染料活性艳红X-3B具有较高降解活性的细菌M7。以M7为出发菌株,经紫外线诱变选育,获得了21株具有抗高浓度X-3B毒性能力的变异菌株UVM7-1～UVM7-21,最终确定UVM7-9是活性最高、脱色能力最强的变异菌株。对UVM7-9在不同的pH值、脱色温度及时间等条件下降解X-3B进行了正交实验,并比较了UVM7-9和M7在处理偶氮染料时的生物活性。结果表明,UVM7-9对含偶氮染料X-3B模拟废水的降解活性优于原始菌株M7,其最佳脱色温度是30℃,pH值为8,24h对X-3B的脱色率达92％以上。
A strain of bacterium M7 which has higher degradation activity to azo dye reactive brilliant red X-3B is isolated and screened from the slurry samples selected from contaminated environment. The 21 strains with strong resistance of the mutant strains of UVM7-1-UVM7-21 to high concentration X-3B coxil ability were obtained with M7 original strains via induced selection by ultraviolet (UV). Finally,the strain of UVM7-9 was determined to be the mutant strain with the highest activity and the strongest decolor ability. The orthogonal experiment was made of UVM7-9 of X-3B degradation in the cases of different pH values, decoloration temperatures and times, etc. The research results show that UVM7-9 has the degradation activity of X-3B simulation wasted water containing azo dyes superior to the original strain M7,whose optimal decoloration time is 30℃,pH value is 8,and X-3B decoloration rate within 24 hours can reach over 92%.
出处
《西安理工大学学报》
CAS
2003年第4期344-347,共4页
Journal of Xi'an University of Technology
基金
陕西省教育厅基金项目(220306)
西安理工大学基金资助项目(210101)
关键词
生物降解
紫外线诱变
偶氮染料
正交实验
biodegradation
ultraviolet mutation
azo dyes
orthogonal experiment
