摘要
目的 建立同时测定复方可待因口服溶液中磷酸可待因、盐酸麻黄碱、盐酸曲普利啶3组分含量的高效液相色谱法。方法 采用Hypersil-BDS CN柱,流动相为乙腈-0.4%乙酸铵溶液-三乙胺(30:70:0.2),流速为1.0 ml/min,检测波长257nm,外标法计算。结果 线性范围分别是磷酸可待因125-1 000 μg/ml,r=0.9999,盐酸麻黄碱75-600μg/ml,r=0.9996;盐酸曲普利啶18-140 μg/ml,r=0.9997。回收率分别为磷酸可待因99.9%、盐酸麻黄碱99.7%、盐酸曲普利啶100.3%。结论 本方法分离效果好,辅料无干扰,快速,简便,适合于该制剂3组分的同时测定。
Objective To establish a method for Simultaneous determination of three constituents codeine phosphate, Ephedrine HCl, triprolidine HCl. in compound codeine phosphate oral solution. Methods HPLC analytical column was Hypersil-BDS CN column. The mobil phase consisted of acetonitrile - 0.4% ammonium acetate-triethylamine(30 : 70 : 0. 2 , v/v/v ) ,The flow rate was 1. 0 ml/min. The UV detection wavelength was 257 nm. Results The linear ranges of three constituents were 125 - 1 000 μg/ml (r =0. 9999) ,75 -600 μg/ml (r =0.9996) ,18 - 140 μg/ml (r=0.9997). The mean recoveries of codeine phosphate, ephedrine HCl, triprolidine HCI were 99. 9% ,99. 7% ,100. 3% ,respectively . Conclusion The method is simple. This method is suitable for the quality control of the compound preperation.
出处
《广东药学》
2003年第6期11-12,共2页
Guangdong Pharmaceutical Journal