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用RISA法评估地下水细菌群落结构差异及变化 被引量:6

Evaluation of Differences and Transition in Groundwater Bacterial Communities by Using Ribosomal Intergenic Spacer Analysis (RISA)
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摘要 提出运用RISA法从细菌群落角度对垃圾填埋场地下水生态系统的空间异质性进行定量分析。RISA图谱的相似度聚类分析表明 :与随时间产生的变化相比 ,细菌群落在空间上的差异更大 ;同批取样不同位置和深度的地下水RISA图谱存在明显差异 ,整体上在 6 0 %~ 70 %相似度聚类。 Ribosomal intergenic spacer analysis (RISA) was applied for quantitative analysis of spatial heterogeneity in landfill leachate-polluted groundwater systems in terms of bacterial community. Computer-assisted cluster analysis of the RISA fingerprints indicated that the spatial differences in the bacterial communities were large relative to the temporal change. There was clear distinctness among the RISA fingerprints of samples collected at the same date but different locations and depths, and cluster analysis grouped them in one large cluster at a level of similarity of 60%~70%.
出处 《微生物学报》 CAS CSCD 北大核心 2004年第5期676-678,共3页 Acta Microbiologica Sinica
基金 国家自然科学基金资助 ( 2 0 3 770 3 0 )~~
关键词 空间异质性 细菌群落 RISA 地下水 Spatial heterogeneity, Bacterial community, RISA, Groundwater
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