摘要
目的 探讨补充不同剂量维生素A(VA)对DNA氧化及烷化损伤的影响。方法 将Wistar大鼠随机分为 4组 ,分别为VA缺乏组 ,及补充VA11 4 3,4 2 86 ,14 2 86 μgRE(视黄醇当量 ) / (kg·d) 3个剂量组。干预时间为 8周。采用单细胞凝胶电泳法 (SCGE)分析比较不同组之间淋巴细胞DNA氧化损伤 ;用高效毛细管电泳法测定尿中O6-甲基鸟嘌呤 (O6-Methyl-guanine ,O6-MeG)的排出量。结果 DNA损伤分析显示VA缺乏组淋巴细胞DNA自发性损伤明显高于VA补充组 (P <0 0 1)。用H2 O2 诱导淋巴细胞氧化损伤时 ,4 2 86 μgRE/ (kg·d)组的淋巴细胞损伤最轻 (P <0 0 1)。缺乏组大鼠尿中的O6-MeG的排出量随缺乏时间的延长明显升高 (P <0 0 1)。 4 2 86 μgRE/ (kg·d)组O6-MeG的排出量无明显改变。 14 2 86 μgRE/ (kg·d)组至第 8周时 ,O6-MeG的排出量分别为VA缺乏组、11 4 3和 4 2 86 μgRE/ (kg·d)组的 1 38倍 (P <0 0 5 )、3 5 7倍 (P <0 0 1)和 11 75倍 (P <0 0 1)。 结论 较长时间VA缺乏可导致机体DNA自发性损伤、H2 O2 诱导的氧化损伤及DNA烷化损伤均明显增加 ;高剂量 14 2 86 μgRE/ (kg·d)VA补充对DNA损伤不但无防护作用 ,反而增加DNA损伤 ;适量摄入 4 2 86 μgRE/ (kg·d)的VA能有效地增强DNA抗氧化和?
Objective To investigate the effect of VA supplement with a different dosage on oxidative DNA daaTage and Oe-MeG adduct in rats.Methods Wistar rats selected for VA supplement trial,were randomly divided into four groups.The first group was no VA supplement as a control,and UTe second,third,forth groups were daily supplemented with 11.43,42.863,142.863、μgRE(Retinol Equivalent)/(kg·d) respectively.DNA oxidative damage in the four groups were detected by single-cell gel electrophoresis (SCGE) and Oe-Methyl-guanine (O 6-MeG) was measured by high performance capillary zone electrophoresis.Results The result of DNA damage showed that intrinsic damage of DNA was more severe in the group of vitamin A deficiency than the groups supplemented with vitamin A (P<0.01).The lowest damage of oxidative DNA damage reduced by H\-2O\-2 was found in 42.863、μg RE/(kg·d) group (P<0.01).O 6-MeG level in the group of vitamin A deficiency increased significantly chaperonage extending of the supplement deficiency(P<0.01).O 6-MeG level was not nearly change in 42.863、μg RE/(kg·d) group in the period of trial.In 142.863、μg RE/(kg·d) group O 6-MeG level which was significantly higher than deficiency and other two groups in the end of the trial,which was 1.38 times in the first(P<0.05),3.57 times in the second (P<0.01) and 11.75 times in the third groups respectively (P<0.01).Conclusion If retinol deaciency for a long time can cause or promote DNA damage.Overake retinol did not pretect DNA damage and could produce a strong poisoning effect.A moderate amount such as 42.863、μg RE/(kg·d) could enhance the ability of antioxidation and mitigating DNA damage.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第8期943-945,共3页
Chinese Journal of Public Health
基金
国家自然科学基金资助 (30 0 70 659)
