摘要
目的 探讨四氧化二氮 (N2 O4)和偏二甲基肼 (UDMH)混合推进剂亚急性中毒时 ,机体氧化指标的变化 ,从而确定氧化损伤在推进剂引起的病理损伤中的作用。 方法 将 4 0只昆明小鼠随机分为对照组和 3个实验组 ,实验组置于染毒柜内 ,吸入含有不同浓度混合剂的挥发气体(1 0mg/m3 ,2h) ,每天 1次 ,连续 4d ,第 5天称重、处死动物。测定血清、肺组织中丙二醛 (MDA)含量和总超氧化物歧化酶 (T SOD)活性。 结果 各染毒组与对照组比较 ,4 0 0mg/m3 染毒组动物体重显著减轻 (P <0 0 1 ) ,血清MDA含量显著减少 (P <0 0 5 ) ,各染毒组肺组织MDA含量均显著减少(P <0 0 1 ) ;90mg/m3 染毒组血清T SOD活性显著降低 (P <0 0 1 ) ,1 0mg/m3 、90mg/m3 组肺组织的T SOD活性显著降低 (P <0 0 1 )。 结论 连续数天高浓度混合推进剂吸入中毒后 ,动物体重减轻 ,提示中毒可引起动物兴奋性增高 ;中、高浓度中毒时自由基产生相对减少 ,氧化指标测定结果提示染毒组小鼠机体未发生显著的脂质过氧化损伤 ;推进剂对机体造成的病理损害中 ,氧化损伤可能不占主导地位 。
Objective To investigate the change of oxidative damage parameters of mice intoxicated by inhaling gas mixture containing N 2O 4 and unsymmetrical dimethyl hydrazine(UDMH), so as to explore the role of oxidative damage in the pathological changes induced by intoxication of the liquid propellant Methods Forty Kunming mice were randomly divided into control group and 3 experimented groups The mice of 3 experimental groups were put into an intoxication cabinet 2 h/d for 4 successive days and the concentration of the mix propellant in the cabinet were 10 mg/m 3, 90 mg/m 3 and 400 mg/m 3 respectively The mice were weighed and killed on the fifth day and the content of malondialdehyde(MDA) and the activity of T SOD in serum and lung tissue were detected Results The weight of mice of 400 mg/m 3 group was significantly lower than that of control group ( P < 0 01) The serum MDA content of 400 mg/m 3 group was significantly lower than that of control group ( P <0 05) The lung tissue MDA contents of all 3 experimental groups were significantly lower than that of control group ( P <0 01). Plasam T SOD activity of 90 mg/m 3 group was signifiany lowered ( P <0 01), and T SOD activity of lung tissue in 10 mg/m 3 and 90 mg/m 3 group was significantly lowered Conclusions The significant loss of weight in mice of high dosage intoxication group indicated the increasing of irritability and metabolic rate of the mice The changes of oxidative damage parameters showed that there were no significant lipid peroxidation damage in the intoxicated mice The mechanism of the liquid propellant intoxication damage remains to be further investigated
出处
《中华航空航天医学杂志》
CSCD
2004年第2期98-101,共4页
Chinese Journal of Aerospace Medicine