摘要
目的利用常温细胞长期培养装置 ,探讨间充质干细胞 (MSCs)无源搭载长期太空飞行的生存条件和培养方法。方法将分离扩增培养的兔MSCs分别接种于培养瓶与常温细胞长期生存装置中 ,在无O2 、CO2 供应 ,以及无更换培养基条件下 ,模拟太空温度变化 ,观察MSCs的长期培养生存状况。结果兔MSCs在无O2 、CO2 供应 ,及无培养基更换条件下 ,4℃ /3 7℃单层培养连续存活至少 15天 ;太空组细胞在常温细胞生存装置中可生存 3 0天 ,两者有显著性差异。结论常温细胞长期生存装置在太空飞行保温温度下可支持兔MSCs连续存活 3
ObjectiveTo investigate the condition and method of a long time mesenchymal stem cell (MSCs) culture with outer space cell living system carried by a recoverable satellite.MethodsMSCs were obtained from 1 week New Zealand rabbits, and seeded to culture flask and outer space cell living system. Under the condition of absence O 2, CO 2 and medium unable changed, influence of monolayer culture and outer space cell living system upon survival of long time MSCs culture in simulated space various temperature was observed.ResultsUnder the condition of absence O 2, CO 2 and medium unable changed, monolayer cultured MSCs were maintained to survive for 15 days, and group of space MSCs were successfully maintained to survive for 30 days in outer space cell living system. There was a significant difference between two methods. ConclusionOuter space cell living system can maintain MSCs to survive for 30 days under space flight temperature.
出处
《中国康复理论与实践》
CSCD
2004年第8期455-457,共3页
Chinese Journal of Rehabilitation Theory and Practice
