摘要
目的 :研究Survivin基因在大肠癌中的表达及临床意义。方法 :应用反转录RT PCR方法 ,检测了 2 9例大肠癌组织中Survivin基因mRNA的表达。结果 :发现正常大肠黏膜组织中Survivin基因不表达 ,肿瘤组织中Survivin基因表达阳性率为 72 .4 % (2 1/ 2 9)。半定量分析结果显示 ,2 9例大肠癌组织中Survivin其表达的平均值为 0 .6 1± 0 .39。Survivin基因表达与淋巴结转移 ,肿瘤分期及远处转移有关。但与年龄 ,肿瘤大小 ,分化程度无关。结论 :提示Survivin基因在大肠癌病程中起重要的作用 ,对筛选大肠癌术后高危转移患者 。
Objective: To explore the correlation between expression of survivin gene in human colorectal adenocarcinoma and its clinical effects. Methods: By using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique, survivin gene expression in 29 previously untreated colorectal adenocarcinoma patients was studyed. The relationships between the expression levels of survivin mRNA and the patient's characteristics, including tumor size,patients' age,lymph node or liver metastisis, presence of invasion degree,clinical stage, and histological grade were analyzed.Results: Survivin gene expression percentage in mucosal tissues of colorectal adenocarcinoma patients were significantly higher than that of normal colorectal mucosa (72.4%,21/29 vs 0/6). The average semi-quantitative analysis result of 29 cases was 0.614±0.39 . Further analysis showed that the expression level of survivin mRNA was significantly associated with clinical stage, lymph node and liver metestasis (P<0.05) respectively. Conclusion:Survivin is expressed in the majority of human colorectal adenocarcinoma and correlates with its clinicopathological factors. These findings suggest that survivin overexpression may play a pivotal role in the progression of colorectal tumors and may provide an important prognostic implication for colorectal carcinomas.It concludes that the survivin mRNA is a defining diagnostic marker for colorectal carcinomas that may also yield prognostic information.
出处
《中国现代普通外科进展》
CAS
2004年第4期226-228,共3页
Chinese Journal of Current Advances in General Surgery
