摘要
从还原NO络合吸收液的混合微生物中分离得到一株克雷伯氏菌(FR-1).考察了氮源量、碳源及其添加量、菌体接种量、pH值和温度对该菌种生长和对Fe+3(EDTA)还原特性的影响.结果表明,菌种FR-1生长适宜的氮源量为100mg/L;以葡萄糖为碳源对微生物生长和Fe+3(EDTA)还原均有利,其添加量为800mg/L时还原效果最好;在接种量为100mg/L时,菌种FR-1生长最好,对Fe+3(EDTA)还原效率随菌体接种量的增加而增大;菌种FR-1生长和对Fe+3(EDTA)还原的最佳温度为40℃;在考察的初始pH值(5.0~8.5)范围内,细菌生长随pH值升高而下降,而对Fe+3(EDTA)的还原随pH值升高而增加.
A bacterial strain FR-1 was isolated from the mixed microbe of the reducing NO chelate absorption solution. The effect of adding the specific property of the bacterial strain and the Fe+3 (EDTA) reduction under the conditions of the amount of nitrogen source, the source and the adding amount of carbon source, the amount of cell inoculation, pH value and temperature were investigated. The amount of nitrogen source of 100mg/L was suitable for FR-1 growth. Glucose used as the carbon source was favorable for microbe growth and Fe+3(EDTA) reduction; and the effect of reduction was the best when the adding amount of glucose was 800mg/L. The FR-1 growth was the best at 100mg/L amount of cell inoculation, Fe+3(EDTA) reduction efficiency was increased with the increase of the amount of cell inoculation. The best temperature for FR-1 growth and Fe+3(EDTA) reduction was 40℃. In the investigated initial pH value range of 5.0~8.5, the cell growth lowered and Fe+3(EDTA) reduction increased with the raise of the pH value.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2004年第4期447-451,共5页
China Environmental Science
基金
国家自然科学基金资助项目(20176052)
教育部留学回国人员科研启动基金资助项目
