摘要
接有芳香烃受体 (AhR)基因和芳香烃受体核转位子蛋白 (ARNT)基因的酵母Saccharomycescerevisiae菌株是测定化学物质的Ah受体效应的方法之一 .本实验通过优化各个实验条件 ,发现用 0 2 %葡萄糖作前培养液培养菌 2 4h ,用 2 %半乳糖作化学物质暴露时的培养液 ,暴露在玻璃管时 ,暴露时间从原来的 1 8h缩短到 8h .并用已经优化的方法测定了六氯苯和五氯苯的Ah受体效应 ,得出六氯苯和五氯苯相对于TCDD的毒性等当量值 (TEF)分别为 0 0 1 86 2 9和 0 0 0 0 2 94 .
A recombinant yeast bioassay,a yeast Saccharomyces cerevisiae, in which the human AhR and ARNT complex are coexpressed,is one of the methods to screen the active AhR agonist.In this study,the original agonist test was modified.The exposure time was reduced from 18 to 8 hours when experiment was under the following conditions:①the yeast was cultured in 0 2% glucose medium for 24 hours;②chemical exposure was carried in 2% galactose medium in glass tube.Finally,the AhR acitivity of hexachlorobenzene and pentachlorobenzene were assessed,and their toxicity equivalent factors were found to be 0 018629 and 0 000294,respectively.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2004年第4期155-158,共4页
Environmental Science
基金
国家杰出青年科学基金项目 ( 4 992 5 10 3 )
国家 863计划项目 ( 2 0 0 2AA60 114 0 6)
