摘要
在研究Fe粉剂量和Fe2 + 浓度对混合细菌产氢发酵的影响基础上 ,确定Fe和Fe2 + 促进混合细菌产氢能力的最佳阈值 ,并对乙醇型发酵菌群在不同Fe粉和Fe2 + 浓度下的产氢量和最大比产氢速率进行考察和对比 .结果表明 ,Fe粉和Fe2 + 对乙醇型发酵菌群的产氢能力均有明显的促进作用 .以葡萄糖为底物 ,投加Fe2 + 试验中 ,Fe2 + 浓度 2 0 0mg/L获得最大产氢量1 4 3 7mL/ g ,较对照组提高 32 % ;Fe2 + 浓度 5 0mg/L获得单位VSS最大比产氢速率 2 1 2mL/ (h·g) ,较对照组提高 33% .投加单质Fe试验中 ,Fe粉剂量 1 0 0 0mg/L获得最大产氢量 1 5 6 1mL/ g ,较对照组提高 4 4 % ;Fe粉剂量 5 0 0mg/L获得单位VSS最大比产氢速率 2 3 5mL/ (h·g) ,较对照组分别提高 4 7% .单质Fe浓度高于 5 0mg/L时 ,对发酵菌群产氢的促进作用要优于同浓度下的Fe2 + .
The effects of Fe and Fe 2+ concentration in the external environment on hydrogen production capacity of ethanol type fermentative microorganism flora were investigated. The optimum range of Fe and Fe 2+ concentration enhanced hydrogen production were obtained and the characteristic of fermentation types at different concentration of Fe and Fe 2+ were discussed. It was demonstrated that both Fe and Fe 2+ were able to enhance the hydrogen production capacity of microorganism flora. The maximum hydrogen production yield of 143.7mL·g -1 glucose and the maximum specific hydrogen production rate 21 2mL·(h·g) -1 were obtained at Fe 2+ concentration of 200mg·L -1 and 50mg·L -1 . The maximum hydrogen production yield of 156 1mL·g -1 glucose and the maximum specific hydrogen production rate 23 5mL·(h·g) -1 were obtained at Fe concentration of 1000mg·L -1 and 500mg·L -1 . At the same concentration, the enhancement of Fe is better than Fe 2+ when the concentration is higher than 50 mg·L -1 . A sequential extraction technique was used to represent arbitrary divisions between different metal forms of Fe 2+ in the mixed culture.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2004年第4期48-53,共6页
Environmental Science
基金
国家重点基础研究发展规划 ( 973 )项目 (G2 0 0 0 0 2 64 0 2 )
国家高技术研究发展计划 ( 863 )项目 ( 2 0 0 3AA5 15 0 3 0 )
关键词
生物制氢
乙醇型发酵
FE
FE^2+
产氢能力
Hydrogen bio-production
ethanol-type fermentation
Fe
Fe 2+
hydrogen production capacity
